Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1042078..1042661 Replicon chromosome
Accession NZ_CP066844
Organism Escherichia coli strain Z30

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag JHL13_RS04960 Protein ID WP_000254750.1
Coordinates 1042326..1042661 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag JHL13_RS04955 Protein ID WP_000581937.1
Coordinates 1042078..1042326 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JHL13_RS04945 (1038417) 1038417..1039718 + 1302 WP_077516534.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
JHL13_RS04950 (1039766) 1039766..1042000 + 2235 WP_000226815.1 GTP pyrophosphokinase -
JHL13_RS04955 (1042078) 1042078..1042326 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
JHL13_RS04960 (1042326) 1042326..1042661 + 336 WP_000254750.1 endoribonuclease MazF Toxin
JHL13_RS04965 (1042733) 1042733..1043524 + 792 WP_001071641.1 nucleoside triphosphate pyrophosphohydrolase -
JHL13_RS04970 (1043752) 1043752..1045389 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
JHL13_RS04975 (1045476) 1045476..1046774 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T185201 WP_000254750.1 NZ_CP066844:1042326-1042661 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T185201 NZ_CP088105:c61402-60974 [Rhizobium ruizarguesonis]
GTGAGACTTCTGCTGGACACCAATGTGCTTTCCGAGGTGACAAGGCCGCGTCCCGATGCACATGTCCTGCAATGGCTGGA
CGATCTCGACGAGGATCGCTCATTCATCAGCGTCGTATCGATCGCGGAGATCCGGCGTGGTGTTGCATTGATGGAAAGCG
GTCGGAAGCGCGACGCGCTGGCTGAATGGCTTGCCCAGGACCTGCCGCAACGCTTCGAGCAGCGTGTCATCCCTGTAGAC
CAGGCGGTTGCGATAACCTGGGGAGACTTGATGGGGCTCGCAAAGCGAAGCGGACGCGGCTTGTCGTCAATGGGTGGCTT
GATTGCCGCCACCGCGATTGCCCACGATCTCACCCTCGCCACCCGTAATATAAAGGATTTCGAGAGCCTTGGGATCGAGC
TTGTCGATCCTTGGATCGAGCGCCCGTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT185201 WP_000581937.1 NZ_CP066844:1042078-1042326 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT185201 NZ_CP088105:c61665-61399 [Rhizobium ruizarguesonis]
ATGCAGCCATCCCGACCAGAGGATAAAAGTTGGACCGTCGCCAGTGCGAAGGCCAAGCTGTCTGAGGTTATCGAACGCGC
GCAAGCAACACCGCAAACGATCATGCGCAATGGTAAACCAAGCGTCGTCGTCGTTTCGGCCGAGGAATGGCAGCGGAAGA
CCGCGCGCAAGGGCACCCTTGCAGAGTTTCTGATGCAATCGCCGCTGCGTGGCGCCGATCTGGACCTCGAACGGCAGCGC
GACGAACCGCGTGATCTCCAGCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References