Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 167117..167729 Replicon chromosome
Accession NZ_CP066844
Organism Escherichia coli strain Z30

Toxin (Protein)


Gene name hicA Uniprot ID U9YXE2
Locus tag JHL13_RS00735 Protein ID WP_000833473.1
Coordinates 167117..167302 (+) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0A1AGA0
Locus tag JHL13_RS00740 Protein ID WP_022646255.1
Coordinates 167319..167729 (+) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JHL13_RS00720 (162583) 162583..163878 + 1296 WP_000985736.1 Fic family protein -
JHL13_RS00725 (163986) 163986..165524 + 1539 WP_000183976.1 aldehyde dehydrogenase AldB -
JHL13_RS00730 (165565) 165565..166644 - 1080 WP_000061477.1 type I restriction enzyme HsdR N-terminal domain-containing protein -
JHL13_RS00735 (167117) 167117..167302 + 186 WP_000833473.1 type II toxin-antitoxin system HicA family toxin Toxin
JHL13_RS00740 (167319) 167319..167729 + 411 WP_022646255.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
JHL13_RS00745 (167850) 167850..169820 - 1971 WP_077517025.1 glycoside hydrolase family 127 protein -
JHL13_RS00750 (169831) 169831..171231 - 1401 WP_000204814.1 MFS transporter -
JHL13_RS00755 (171457) 171457..172272 + 816 WP_000891820.1 AraC family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6800.89 Da        Isoelectric Point: 11.7053

>T185197 WP_000833473.1 NZ_CP066844:167117-167302 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Download         Length: 186 bp

>T185197 NZ_CP088100:c2160381-2159956 [Bradyrhizobium japonicum]
GTGAACCTGCTCCTCGACACCAACGTGCTGTCGGAGGTTCAGCGGCCGGCGCCTTCCCCGAAAGTCCTGGCATGGCTCGA
TACGGTCGATGAGGATCGCACGTTCATCAGCGTTGCTTCGATCGCCGAGCTTCGCCGCGGCATCGCCCTGCTGGACGATG
GCCGTCGGCGCGCCGCACTGGTCGCTTGGCTCGCCCATGACCTGCCGGCTCGGTTCGCGCAGCGCATCCTGCCGATCGAC
CACGCGGTCGCTGAGCGCTGGGGCGACCTGATGGCGCAGAGCCGTCGCAATGGCGTTGCGTTGTCCGTGATGGACGGCTT
CTTTGCGGCCACCGCGCTCGCCAACGATCTCACGCTCGTCACACGCAATGTGAAAGATTTTGCGGCCTTCGGCGTTCCGC
TGCTCGACCCATGGAGCGACGCCTAA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 15214.08 Da        Isoelectric Point: 4.4482

>AT185197 WP_022646255.1 NZ_CP066844:167319-167729 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALAAHFETLCEMDEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Download         Length: 411 bp

>AT185197 NZ_CP088100:c2160659-2160378 [Bradyrhizobium japonicum]
ATGACAGACCGCAGCACCGCCCCCGATTCGCCCCCGACTGACACGTGGACGCTCGCCAATGCCAAGGCACGCCTGTCCGA
GGTGGTCGATCGCGCCCAAACTGGCCCCCAGATCATCACCCGACACGGCAGGCCCAATGCGGTTGTCGTTTCCGCGGATG
AATGGGCGCGAAAGACGGCACGCAAAGGCACGCTAGCTGAATTCCTGCTGGCTTCGCCGCTGCGCGGTGCCGACCTCGAA
CTTGAACGGGTGCAGGACGAACCGCGCGACGAGATGCCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9YXE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0A1AGA0

References