Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 41201..41748 Replicon plasmid pIncF4-A-B1
Accession NZ_CP066841
Organism Escherichia coli strain Ec-RL2-1X

Toxin (Protein)


Gene name parE Uniprot ID G7QEJ9
Locus tag JH656_RS24000 Protein ID WP_001384452.1
Coordinates 41201..41479 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A3A1Q2G3
Locus tag JH656_RS24005 Protein ID WP_042021804.1
Coordinates 41479..41748 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JH656_RS23965 (36304) 36304..37230 + 927 WP_080077783.1 hypothetical protein -
JH656_RS23970 (37280) 37280..37534 + 255 WP_059291112.1 hypothetical protein -
JH656_RS23975 (38032) 38032..38376 + 345 WP_087601730.1 abortive infection protein -
JH656_RS23980 (38550) 38550..39551 - 1002 WP_000774874.1 DUF4238 domain-containing protein -
JH656_RS23985 (39902) 39902..40159 + 258 WP_000371888.1 hypothetical protein -
JH656_RS23990 (40159) 40159..40749 + 591 WP_000194553.1 hypothetical protein -
JH656_RS23995 (40769) 40769..41116 - 348 WP_024148462.1 DUF6404 family protein -
JH656_RS24000 (41201) 41201..41479 - 279 WP_001384452.1 type II toxin-antitoxin system toxin YacB Toxin
JH656_RS24005 (41479) 41479..41748 - 270 WP_042021804.1 type II toxin-antitoxin system antitoxin YacA Antitoxin
JH656_RS24010 (41803) 41803..41961 - 159 WP_000829588.1 hypothetical protein -
JH656_RS24015 (42664) 42664..43521 - 858 WP_001697864.1 incFII family plasmid replication initiator RepA -
JH656_RS24645 (43514) 43514..43588 - 75 WP_001375168.1 RepA leader peptide Tap -
JH656_RS24030 (43824) 43824..44081 - 258 WP_000083826.1 replication regulatory protein RepA -
JH656_RS24035 (44321) 44321..44911 - 591 WP_000766805.1 DUF2726 domain-containing protein -
JH656_RS24040 (44949) 44949..45158 - 210 WP_001298565.1 hemolysin expression modulator Hha -
JH656_RS24045 (45204) 45204..45665 - 462 WP_001233838.1 thermonuclease family protein -
JH656_RS24050 (45910) 45910..46122 - 213 WP_001309245.1 ANR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iroB / iroC / iroD / iroE / iroN 1..113350 113350


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10762.38 Da        Isoelectric Point: 8.4615

>T185193 WP_001384452.1 NZ_CP066841:c41479-41201 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Download         Length: 279 bp

>T185193 NZ_CP088097:61325-61597 [Rhizobium leguminosarum bv. viciae]
ATGAAATTGCTATGGACCCCTAATTCCTTGGAAGAGTACGAATACTGGCAGAAAAACGACCAGAAGATGGTCGAGAAAAT
CAACGAACTCCTGAAGGATACAAAAAGGTCACCCTTTAAAGGTCTGGGCAAGCCAGAGCCACTGAAGGGCGATCTATCCG
GATTTTGGTCAAGGCGCATACACGGAGAGCATCGGCTCGTCTACTGCGTCACTGGCAAGGGTGCCAACCAACAGCTGGAA
ATCATCCAGTGCCGATTTCACTACGAAAAATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10122.48 Da        Isoelectric Point: 10.2879

>AT185193 WP_042021804.1 NZ_CP066841:c41748-41479 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLGRGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT185193 NZ_CP088097:61072-61323 [Rhizobium leguminosarum bv. viciae]
ATGGCCCATGTTCGTTTGACGGAGTTCCGGCAAAACATTGCAACCTATTTTGATCAAGTCCTCTCTTCTCGGGCTCCTCT
GCTTGTGACCCGTCAAGGCTCGGAAGCAATGGTCCTGGTTGCCGAAGGCGAGTACGAGAGTATGCAAGAGACGCTACACC
TGCTCTCGACGCCTGCTAATTCTGCCCGGCTACGCGAAAGTATAGGACAGCTTCGAAAGGGGCAGGTGATAGAGAGCGAT
CCAACTCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CL36

Antitoxin

Source ID Structure
AlphaFold DB A0A3A1Q2G3

References