Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | parDE/RelE-RHH |
| Location | 41201..41748 | Replicon | plasmid pIncF4-A-B1 |
| Accession | NZ_CP066841 | ||
| Organism | Escherichia coli strain Ec-RL2-1X | ||
Toxin (Protein)
| Gene name | parE | Uniprot ID | G7QEJ9 |
| Locus tag | JH656_RS24000 | Protein ID | WP_001384452.1 |
| Coordinates | 41201..41479 (-) | Length | 93 a.a. |
Antitoxin (Protein)
| Gene name | parD | Uniprot ID | A0A3A1Q2G3 |
| Locus tag | JH656_RS24005 | Protein ID | WP_042021804.1 |
| Coordinates | 41479..41748 (-) | Length | 90 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JH656_RS23965 (36304) | 36304..37230 | + | 927 | WP_080077783.1 | hypothetical protein | - |
| JH656_RS23970 (37280) | 37280..37534 | + | 255 | WP_059291112.1 | hypothetical protein | - |
| JH656_RS23975 (38032) | 38032..38376 | + | 345 | WP_087601730.1 | abortive infection protein | - |
| JH656_RS23980 (38550) | 38550..39551 | - | 1002 | WP_000774874.1 | DUF4238 domain-containing protein | - |
| JH656_RS23985 (39902) | 39902..40159 | + | 258 | WP_000371888.1 | hypothetical protein | - |
| JH656_RS23990 (40159) | 40159..40749 | + | 591 | WP_000194553.1 | hypothetical protein | - |
| JH656_RS23995 (40769) | 40769..41116 | - | 348 | WP_024148462.1 | DUF6404 family protein | - |
| JH656_RS24000 (41201) | 41201..41479 | - | 279 | WP_001384452.1 | type II toxin-antitoxin system toxin YacB | Toxin |
| JH656_RS24005 (41479) | 41479..41748 | - | 270 | WP_042021804.1 | type II toxin-antitoxin system antitoxin YacA | Antitoxin |
| JH656_RS24010 (41803) | 41803..41961 | - | 159 | WP_000829588.1 | hypothetical protein | - |
| JH656_RS24015 (42664) | 42664..43521 | - | 858 | WP_001697864.1 | incFII family plasmid replication initiator RepA | - |
| JH656_RS24645 (43514) | 43514..43588 | - | 75 | WP_001375168.1 | RepA leader peptide Tap | - |
| JH656_RS24030 (43824) | 43824..44081 | - | 258 | WP_000083826.1 | replication regulatory protein RepA | - |
| JH656_RS24035 (44321) | 44321..44911 | - | 591 | WP_000766805.1 | DUF2726 domain-containing protein | - |
| JH656_RS24040 (44949) | 44949..45158 | - | 210 | WP_001298565.1 | hemolysin expression modulator Hha | - |
| JH656_RS24045 (45204) | 45204..45665 | - | 462 | WP_001233838.1 | thermonuclease family protein | - |
| JH656_RS24050 (45910) | 45910..46122 | - | 213 | WP_001309245.1 | ANR family transcriptional regulator | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | sitABCD | iroB / iroC / iroD / iroE / iroN | 1..113350 | 113350 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 93 a.a. Molecular weight: 10762.38 Da Isoelectric Point: 8.4615
>T185193 WP_001384452.1 NZ_CP066841:c41479-41201 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP
Download Length: 279 bp
>T185193 NZ_CP088097:61325-61597 [Rhizobium leguminosarum bv. viciae]
ATGAAATTGCTATGGACCCCTAATTCCTTGGAAGAGTACGAATACTGGCAGAAAAACGACCAGAAGATGGTCGAGAAAAT
CAACGAACTCCTGAAGGATACAAAAAGGTCACCCTTTAAAGGTCTGGGCAAGCCAGAGCCACTGAAGGGCGATCTATCCG
GATTTTGGTCAAGGCGCATACACGGAGAGCATCGGCTCGTCTACTGCGTCACTGGCAAGGGTGCCAACCAACAGCTGGAA
ATCATCCAGTGCCGATTTCACTACGAAAAATAG
ATGAAATTGCTATGGACCCCTAATTCCTTGGAAGAGTACGAATACTGGCAGAAAAACGACCAGAAGATGGTCGAGAAAAT
CAACGAACTCCTGAAGGATACAAAAAGGTCACCCTTTAAAGGTCTGGGCAAGCCAGAGCCACTGAAGGGCGATCTATCCG
GATTTTGGTCAAGGCGCATACACGGAGAGCATCGGCTCGTCTACTGCGTCACTGGCAAGGGTGCCAACCAACAGCTGGAA
ATCATCCAGTGCCGATTTCACTACGAAAAATAG
Antitoxin
Download Length: 90 a.a. Molecular weight: 10122.48 Da Isoelectric Point: 10.2879
>AT185193 WP_042021804.1 NZ_CP066841:c41748-41479 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLGRGDVLPHDMVESSAAAWRD
EMSRKVAGK
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLGRGDVLPHDMVESSAAAWRD
EMSRKVAGK
Download Length: 270 bp
>AT185193 NZ_CP088097:61072-61323 [Rhizobium leguminosarum bv. viciae]
ATGGCCCATGTTCGTTTGACGGAGTTCCGGCAAAACATTGCAACCTATTTTGATCAAGTCCTCTCTTCTCGGGCTCCTCT
GCTTGTGACCCGTCAAGGCTCGGAAGCAATGGTCCTGGTTGCCGAAGGCGAGTACGAGAGTATGCAAGAGACGCTACACC
TGCTCTCGACGCCTGCTAATTCTGCCCGGCTACGCGAAAGTATAGGACAGCTTCGAAAGGGGCAGGTGATAGAGAGCGAT
CCAACTCTCTGA
ATGGCCCATGTTCGTTTGACGGAGTTCCGGCAAAACATTGCAACCTATTTTGATCAAGTCCTCTCTTCTCGGGCTCCTCT
GCTTGTGACCCGTCAAGGCTCGGAAGCAATGGTCCTGGTTGCCGAAGGCGAGTACGAGAGTATGCAAGAGACGCTACACC
TGCTCTCGACGCCTGCTAATTCTGCCCGGCTACGCGAAAGTATAGGACAGCTTCGAAAGGGGCAGGTGATAGAGAGCGAT
CCAACTCTCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CL36 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3A1Q2G3 |