Detailed information of TA system TA185192

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 101591..102192
• Replicon: plasmid pHI1B-o111
• Accession: NZ_CP066840
• Organism: Escherichia coli strain Ec-RL2-1X

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: JH656_RS23645
• Protein ID: WP_072731915.1
• Coordinates: 101591..101971 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: JH656_RS23650
• Protein ID: WP_001190712.1
• Coordinates: 101971..102192 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH656_RS23605 (JH656_23410)	96700..96921	-	222	WP_001697576.1	hypothetical protein	-
JH656_RS23610 (JH656_23415)	97863..98714	+	852	WP_089586253.1	phage repressor protein C1	-
JH656_RS23615	98747..99079	-	333	WP_223715978.1	phage antirepressor KilAC domain-containing protein	-
JH656_RS23620	99200..99550	-	351	Protein_101	ORF6N domain-containing protein	-
JH656_RS23625 (JH656_23425)	99859..100059	-	201	WP_089586254.1	host cell division inhibitor Icd-like protein	-
JH656_RS23630 (JH656_23430)	100065..100247	-	183	Protein_103	phage antirepressor Ant	-
JH656_RS23635 (JH656_23435)	100336..101379	-	1044	WP_211131004.1	DUF968 domain-containing protein	-
JH656_RS23640 (JH656_23440)	101407..101586	-	180	WP_000113018.1	hypothetical protein	-
JH656_RS23645 (JH656_23445)	101591..101971	-	381	WP_072731915.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
JH656_RS23650 (JH656_23450)	101971..102192	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JH656_RS23655 (JH656_23455)	102375..103931	+	1557	WP_089586248.1	type I restriction-modification system subunit M	-
JH656_RS23660 (JH656_23460)	103928..105067	+	1140	WP_089586244.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(B) / ant(3'')-Ia / aac(3)-VIa / qacE / sul1	-	1..136209	136209

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13631.32 Da
• Isoelectric Point: 5.1514

>T185192 WP_072731915.1 NZ_CP066840:c101971-101591 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSSE

Nucleotide

• Length: 381 bp

>T185192 NZ_CP088096:c269315-268890 [Rhizobium leguminosarum bv. viciae]
ATGATCCTGATCGATACCAACGTCATCTCGGAGCCATGGAAGCCCACACCCGATAATCAGGTACTGGCGTGGATCGATGC
CCAAGCAGTGGAAACGCTGTTCGTGTCGGCGATTACAGTAGCTGAGTTACGGTTCGGCATTGCCGCGATGCCTAGCGGAA
GACGAAAGACCGTCCTCTACGATCGACTAGAACAGGAAGTTCTGCCTCTATTTTCAGGGCGCGTTCTATCCTTCGACATG
GCTGCGTCACAGAGCTATTCGGAATTGATGGCGCGAGCACGCGTAGCAGGTCGGGCAATCGGTAAAGCAGATGCCTATAT
CGCCGCAACTGCCGCGGCGAACGGATTTATTGTCGCCACCCGCGACACTAGTCCCTTCGAAGCCGCAGGACTTAACGTTA
TCAACCCGTGGTACGCACCAACATAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT185192 WP_001190712.1 NZ_CP066840:c102192-101971 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT185192 NZ_CP088096:c269566-269312 [Rhizobium leguminosarum bv. viciae]
ATGGCTGCAGTTACAATACGCAATTTGTCTGACGAGACACATCGTGCCTTAAGAGTGAGGGCAGCTCATCACGGCCGCAG
TACAGAAGCAGAAATCCGTGACATCCTTGAAACGGCGGTACGGCCAGCTGAGCGCGTCATGTTGGGCTCGTTGCTAGCAT
CGATCGGGCGCGAGGCAGGGCTTTCCAACAACGACGTGGATAATCTGCAGCTCGTCCGAGACAAAGCGCCTGCGGAACCT
ATGAGTTTCGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6