Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 43266..43801 Replicon plasmid pHI1B-o111
Accession NZ_CP066840
Organism Escherichia coli strain Ec-RL2-1X

Toxin (Protein)


Gene name relE Uniprot ID A0A0K4CX11
Locus tag JH656_RS23335 Protein ID WP_000222771.1
Coordinates 43266..43553 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID T1PXV2
Locus tag JH656_RS23340 Protein ID WP_001139206.1
Coordinates 43550..43801 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JH656_RS23320 (JH656_23125) 38891..42406 + 3516 WP_072752622.1 autotransporter outer membrane beta-barrel domain-containing protein -
JH656_RS23325 (JH656_23130) 42581..42786 - 206 Protein_42 hypothetical protein -
JH656_RS23330 42785..42898 + 114 Protein_43 alkaline phosphatase -
JH656_RS23335 (JH656_23140) 43266..43553 - 288 WP_000222771.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JH656_RS23340 (JH656_23145) 43550..43801 - 252 WP_001139206.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JH656_RS23345 (JH656_23150) 44460..45668 + 1209 WP_001352368.1 IS4-like element ISVsa5 family transposase -
JH656_RS23350 (JH656_23155) 45684..45803 - 120 Protein_47 LysR family transcriptional regulator -
JH656_RS23355 (JH656_23160) 46034..47071 - 1038 WP_242562025.1 sodium/glutamate symporter -
JH656_RS23360 (JH656_23165) 47068..48276 - 1209 WP_001339197.1 IS4-like element ISVsa5 family transposase -
JH656_RS23365 (JH656_23170) 48386..48577 - 192 Protein_50 sodium/glutamate symporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(B) / ant(3'')-Ia / aac(3)-VIa / qacE / sul1 - 1..136209 136209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11116.17 Da        Isoelectric Point: 10.5833

>T185191 WP_000222771.1 NZ_CP066840:c43553-43266 [Escherichia coli]
MTYTVKFRDDALKEWLRLDKTIQQQFAKKLKKCSENPHIASAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYHLASERMK

Download         Length: 288 bp

>T185191 NZ_CP088096:c57913-57485 [Rhizobium leguminosarum bv. viciae]
GTGAGACTTCTGCTGGACACCAATGTGCTTTCCGAGGTGACAAGGCCGCGTCCCGATGCACGTGTCCTGCAATGGCTGGA
CAGTCTCGACGAGGATCGCTCATTCATCAGCACCGTATCGATCGCGGAGATCCGGCGTGGTGTTGCATTGATGGAAAGCG
GCCGGAAGCGCGACGCGCTGGCTGAATGGCTTGCACAGGACCTGCCGCAGCGCTTCGAGCAGCGCGTCACCCCTGTCGAC
CAGCCGGTTGCGATAGTCTGGGGCGACCTGATGGGGCTCGCAAAGCGAAGCGGACGCGGCTTGTCGTCAATGGACGGCTT
GATTGCCGCCACCGCGATTGCTCACGATCTCACTCTCGCCACCCGTAATATAAAGGATTTCGAAGGTTTTGGGATCGAGC
TTGTCGATCCTTGGATAGAGCGCCCATGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9171.39 Da        Isoelectric Point: 4.0927

>AT185191 WP_001139206.1 NZ_CP066840:c43801-43550 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSSQPLHDVDLD
SYL

Download         Length: 252 bp

>AT185191 NZ_CP088096:c58176-57910 [Rhizobium leguminosarum bv. viciae]
ATGCAGCCATCACGACCAGAGGATAAAAATTGGACCGTCGCCAGTGCGAAGGCCAAGCTGTCTGAGGTTATCGAACGCGC
GCAAGCAACGCCGCAAACGATCATGCGCAATGGTAAACCAAGCGTCGTCGTCGTTTCGGCCGAAGAATGGCAGCGGAAGA
CCGCGCGCAAGGGCACCCTTGCAGAGTTTCTGATGCAATCGCCGCTGAGTGGCGCCGATCTGGACCTCGGACGACAGCGC
GACGAACCGCGTGATCTCCAGCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K4CX11

Antitoxin

Source ID Structure
AlphaFold DB T1PXV2

References