Detailed information of TA system TA185190

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4247396..4248210
• Replicon: chromosome
• Accession: NZ_CP066839
• Organism: Escherichia coli strain Ec-RL2-1X

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: JH656_RS20890
• Protein ID: WP_001054376.1
• Coordinates: 4247396..4247653 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: JH656_RS20895
• Protein ID: WP_001309181.1
• Coordinates: 4247665..4248210 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH656_RS20865 (4242684)	4242684..4243790	+	1107	WP_023568191.1	N-acetylneuraminate epimerase	-
JH656_RS20870 (4243855)	4243855..4244835	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
JH656_RS20875 (4244945)	4244945..4245150	+	206	Protein_4087	HNH endonuclease	-
JH656_RS20880 (4245418)	4245418..4246658	-	1241	Protein_4088	helicase YjhR	-
JH656_RS20885 (4246774)	4246774..4246905	+	132	WP_001309182.1	hypothetical protein	-
JH656_RS20890 (4247396)	4247396..4247653	+	258	WP_001054376.1	YjhX family toxin	Toxin
JH656_RS20895 (4247665)	4247665..4248210	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
JH656_RS20900 (4248266)	4248266..4249012	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
JH656_RS20905 (4249181)	4249181..4249399	+	219	Protein_4093	hypothetical protein	-
JH656_RS20910 (4249437)	4249437..4249553	+	117	Protein_4094	VOC family protein	-
JH656_RS20915 (4249798)	4249798..4250919	+	1122	WP_000010829.1	M42 family metallopeptidase	-
JH656_RS20920 (4250916)	4250916..4251194	+	279	WP_000722973.1	PTS sugar transporter subunit IIB SgcB	-
JH656_RS20925 (4251206)	4251206..4252519	+	1314	WP_000460843.1	PTS sugar transporter subunit IIC SgcC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	fimE / fimB	4238816..4305904	67088

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T185190 WP_001054376.1 NZ_CP066839:4247396-4247653 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T185190 NZ_CP088095:c166666-166418 [Rhizobium leguminosarum bv. viciae]
CTGCGCAAGCTGAAGGATCGGAATGCAAGAGACCGAATTGTCACGCGCATCGTTCGTGCGGAGGCTGGCAATTTGGGTGA
TCACAAATTCTTCGATGGCATCGGGGAATTGAGGCTCGACTACGGTCCTGGGTACCGTTTGTATTTTGCAATGCAGGGCA
GCGTTGTCGTTATTCTCCTGTGCGGGGGCGACAAGGGCAGCCAGGATCGGGACATCAAGCGAGCAATCGAAATGTCCAAG
GAGATATGA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT185190 WP_001309181.1 NZ_CP066839:4247665-4248210 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT185190 NZ_CP088095:c166416-166093 [Rhizobium leguminosarum bv. viciae]
ATGGCGCTTGAAATCATCCCTTTTGACGCAACCGAGTTTCTGGATACCGAAGAATCTCAGGCCCATCTCATTGAAGATGC
GTTTGAAAGCGGTGATGCAGGTTATATCGCGCACGCATTGGGCGTTGTTGCGAAAGCTCGTGGTATGTCACAGGTCGCTC
GGGATGCCGGCGTTACCCGTGAGGCTCTCTACAAATCGCTCAGTGAAAAAGGCGACCCAAAGCTTTCAACGCTCATCGGT
GTAGCCGCAGCTCTCGGATACAAGCTGACAGCGACGCGTGTGGATGATTCAGATACACACGAGGACGCGCGTCGATCTGC
TTAG

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8