Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4207599..4208175 Replicon chromosome
Accession NZ_CP066839
Organism Escherichia coli strain Ec-RL2-1X

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag JH656_RS20705 Protein ID WP_001295743.1
Coordinates 4207888..4208175 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag JH656_RS20700 Protein ID WP_000063148.1
Coordinates 4207599..4207901 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JH656_RS20680 (4203360) 4203360..4203902 + 543 WP_000934391.1 hypothetical protein -
JH656_RS20685 (4204195) 4204195..4206345 + 2151 WP_001298933.1 pyruvate/proton symporter BtsT -
JH656_RS20690 (4206395) 4206395..4206598 + 204 WP_000467859.1 YbdD/YjiX family protein -
JH656_RS20695 (4206609) 4206609..4207565 + 957 WP_089625875.1 GTPase -
JH656_RS20700 (4207599) 4207599..4207901 - 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
JH656_RS20705 (4207888) 4207888..4208175 - 288 WP_001295743.1 BrnT family toxin Toxin
JH656_RS20710 (4208372) 4208372..4209538 + 1167 WP_000800831.1 restriction endonuclease -
JH656_RS20715 (4209602) 4209602..4211221 + 1620 WP_089625877.1 class I SAM-dependent DNA methyltransferase -
JH656_RS20720 (4211211) 4211211..4212524 + 1314 WP_097177238.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T185189 WP_001295743.1 NZ_CP066839:c4208175-4207888 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T185189 NZ_CP088095:c166416-166093 [Rhizobium leguminosarum bv. viciae]
ATGGCGCTTGAAATCATCCCTTTTGACGCAACCGAGTTTCTGGATACCGAAGAATCTCAGGCCCATCTCATTGAAGATGC
GTTTGAAAGCGGTGATGCAGGTTATATCGCGCACGCATTGGGCGTTGTTGCGAAAGCTCGTGGTATGTCACAGGTCGCTC
GGGATGCCGGCGTTACCCGTGAGGCTCTCTACAAATCGCTCAGTGAAAAAGGCGACCCAAAGCTTTCAACGCTCATCGGT
GTAGCCGCAGCTCTCGGATACAAGCTGACAGCGACGCGTGTGGATGATTCAGATACACACGAGGACGCGCGTCGATCTGC
TTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT185189 WP_000063148.1 NZ_CP066839:c4207901-4207599 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT185189 NZ_CP088095:c166666-166418 [Rhizobium leguminosarum bv. viciae]
CTGCGCAAGCTGAAGGATCGGAATGCAAGAGACCGAATTGTCACGCGCATCGTTCGTGCGGAGGCTGGCAATTTGGGTGA
TCACAAATTCTTCGATGGCATCGGGGAATTGAGGCTCGACTACGGTCCTGGGTACCGTTTGTATTTTGCAATGCAGGGCA
GCGTTGTCGTTATTCTCCTGTGCGGGGGCGACAAGGGCAGCCAGGATCGGGACATCAAGCGAGCAATCGAAATGTCCAAG
GAGATATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References