185186

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3730910..3731747 Replicon chromosome
Accession NZ_CP066839
Organism Escherichia coli strain Ec-RL2-1X

Toxin (Protein)


Gene name itaT Uniprot ID A0A140NB16
Locus tag JH656_RS18400 Protein ID WP_000227786.1
Coordinates 3731205..3731747 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag JH656_RS18395 Protein ID WP_001297137.1
Coordinates 3730910..3731221 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JH656_RS18370 (3725930) 3725930..3726877 + 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -
JH656_RS18375 (3726899) 3726899..3728890 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
JH656_RS18380 (3728880) 3728880..3729494 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
JH656_RS18385 (3729494) 3729494..3729823 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
JH656_RS18390 (3729835) 3729835..3730725 + 891 WP_000971336.1 heme o synthase -
JH656_RS18395 (3730910) 3730910..3731221 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
JH656_RS18400 (3731205) 3731205..3731747 + 543 WP_000227786.1 GNAT family N-acetyltransferase Toxin
JH656_RS18405 (3731803) 3731803..3732738 - 936 WP_001297127.1 tetratricopeptide repeat protein -
JH656_RS18410 (3733155) 3733155..3734519 + 1365 WP_001000978.1 MFS transporter -
JH656_RS18415 (3734647) 3734647..3735138 - 492 WP_001138904.1 nucleotide binding protein YajQ -
JH656_RS18420 (3735306) 3735306..3736217 + 912 WP_000705853.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19794.98 Da        Isoelectric Point: 8.3395

>T185186 WP_000227786.1 NZ_CP066839:3731205-3731747 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T185186 NZ_CP088092:61873-62166 [Rhizobium laguerreae]
ATGAAATTGCAATGGACCCCTAATTCCTGGGAAGAGTACGAATACTGGCAGAAAAACGACCAGAAGATGGTCGAGAGAAT
CGACGAACTCCTGAAGGATACAAAAAGGTCACCCTTTAAAGGTCTGGGCAAGCCAGAGCCACTGAAGGGAGATCTATCCG
GATTTTGGTCAAGGCGCATCCTCGGAGAGCATCGACTCGTCTACTGCGTGACTGGCAAGGGTTCCAACCAACAGCTGGAA
ATCATCCAGTGCCGATTTCATTACGAAAAATGGGTTCTTGCGCAGTGCAGTTGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT185186 WP_001297137.1 NZ_CP066839:3730910-3731221 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT185186 NZ_CP088092:61620-61871 [Rhizobium laguerreae]
ATGGCCCACGTTCGCTTGACGGAGTTCCGCCAAAACATTGCAACCTATTTTGATCAAGTCCTCTCTTCTCGGGCTCCCCT
GCTAGTGACCCGTCAAGGCTCGGAAGCGATGGTCTTGGTTGCCGAAGGCGAGTACGAGAGTATGCAAGAGACGCTACACC
TGCTTTCGACGCCCGCTAATTCTGCCCGGCTACGCGAAAGTCTCGGACAGCTTCGAAAGGGGCAGGTGGTAGAGAGCGAT
CCCACTCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140NB16

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References