Detailed information of TA system TA185185

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2700639..2701277
• Replicon: chromosome
• Accession: NZ_CP066839
• Organism: Escherichia coli strain Ec-RL2-1X

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: JH656_RS13255
• Protein ID: WP_000813794.1
• Coordinates: 2701101..2701277 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JH656_RS13250
• Protein ID: WP_001270286.1
• Coordinates: 2700639..2701055 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH656_RS13230 (2695791)	2695791..2696732	-	942	WP_001251313.1	ABC transporter permease	-
JH656_RS13235 (2696733)	2696733..2697746	-	1014	WP_242561906.1	ABC transporter ATP-binding protein	-
JH656_RS13240 (2697764)	2697764..2698909	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
JH656_RS13245 (2699154)	2699154..2700560	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
JH656_RS13250 (2700639)	2700639..2701055	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JH656_RS13255 (2701101)	2701101..2701277	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JH656_RS13260 (2701499)	2701499..2701729	+	231	WP_000494244.1	YncJ family protein	-
JH656_RS13265 (2701821)	2701821..2703782	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JH656_RS13270 (2703855)	2703855..2704391	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
JH656_RS13275 (2704483)	2704483..2705658	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T185185 WP_000813794.1 NZ_CP066839:c2701277-2701101 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T185185 NZ_CP088091:724474-724971 [Rhizobium laguerreae]
GTGACCTTAAGCGTACCCGGGCCACTTGCTGACCATCACGAGCTAGCCGAATTCCATTCTGGTGTCCCTGAGCTTGACGA
TTGGCTGCGTCTGCGCGCCCGCGCCAATCAGGCGAGCGGCGCATCGCGAACCTTCGTCGTGTGTGAGGCCAACCGCGTCA
TCGCCTATTACGCCCTCGCTTCCGGCGCGGTAAAGCAATCGGAAGCGCCGGGACGTTTCCGGCGGAACATGCCTGATCCG
ATACCAGTCGCCGTGCTTGGACGCCTTGCCATTGATCAAACCTATCAGGGACGCGGTTTCGGCAGGGCGTTAGTCCGCGA
CGCAGGTCTGCGCTTGATCAATGCCGCCGAGATACTTGGGATTCGCGGCGTTTTGGTCCATGCGATATCTGATGACGCAC
GAGCCTTCTACGAAGCGGTTGGCTTCCTGCCTTCCCCGTCCGACCCCATGATGCTGCTGGTCGGGTTGCATGACCTCAAC
AACGCGCTGACCTCCTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT185185 WP_001270286.1 NZ_CP066839:c2701055-2700639 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT185185 NZ_CP088091:724181-724477 [Rhizobium laguerreae]
ATGCCGACCTCGCACCCAAGCAAACGCCCGGACGCAAGCGTGGCGCTGAATATGCGCATTAAGCCTGCGACGCGCAACTT
GATTGACCGCGCCGCTGAAATGCTAGGCAAGACACGCACAGATTTTATGCTGGAAGCTTCCGAGCGCCGTGCCGAGGAAG
TACTACTTGACCGGACGATCTTCACTGTCAGCGCAGAAGTCTACGCAGAGTATCTGGCCCGGCTCGATGCGCCAACACAG
CCCAATGAGCGTCTTAAGCGCACCATGTCGACCAAAGCGCCGTGGGATGAGGTGTGA

Structures

Toxin

• PDB: 6HPB