Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2611266..2612854 Replicon chromosome
Accession NZ_CP066839
Organism Escherichia coli strain Ec-RL2-1X

Toxin (Protein)


Gene name hipA Uniprot ID S1ELM3
Locus tag JH656_RS12870 Protein ID WP_001125439.1
Coordinates 2611532..2612854 (+) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID -
Locus tag JH656_RS12865 Protein ID WP_001301023.1
Coordinates 2611266..2611532 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JH656_RS12840 (2606522) 2606522..2606731 - 210 Protein_2509 (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase -
JH656_RS12845 (2606755) 2606755..2607630 - 876 WP_167814369.1 3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase -
JH656_RS12850 (2607657) 2607657..2608679 - 1023 WP_000172488.1 autoinducer 2 ABC transporter substrate-binding protein LsrB -
JH656_RS12855 (2608691) 2608691..2609467 - 777 Protein_2512 histidine kinase -
JH656_RS12860 (2609463) 2609463..2611043 + 1581 Protein_2513 autotransporter outer membrane beta-barrel domain-containing protein -
JH656_RS12865 (2611266) 2611266..2611532 + 267 WP_001301023.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
JH656_RS12870 (2611532) 2611532..2612854 + 1323 WP_001125439.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
JH656_RS12875 (2613166) 2613166..2613329 + 164 Protein_2516 AraC family transcriptional regulator -
JH656_RS12880 (2613690) 2613690..2614400 + 711 Protein_2517 fimbria/pilus periplasmic chaperone -
JH656_RS12885 (2614442) 2614442..2617092 + 2651 Protein_2518 fimbrial biogenesis outer membrane usher protein -
JH656_RS12890 (2617106) 2617106..2617636 + 531 WP_000876763.1 fimbrial protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2605205..2606413 1208


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(149-401)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49275.82 Da        Isoelectric Point: 8.2482

>T185184 WP_001125439.1 NZ_CP066839:2611532-2612854 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Download         Length: 1323 bp

>T185184 NZ_CP088091:c722053-721610 [Rhizobium laguerreae]
GTGACTTTCCTGCTCGACGTCAATGTGCTGATTGCCTTAATCGATCCGGGCCATGTAGCCCATGATGATGCGCACGCATG
GTTCGCGGCGATAGGTCAGAGCGCGTGGGCTACCTGCCCCATTACCGAAAATGGTGTCATCCGGATTGTCGGCAACCCCA
AATATCCCAACTCTCCCGGTTCCCCGTCACTCGTGATGGAAATTGTCCGCAAATTGCGGTCGCTCCCCGGTCACAGTTTT
TGGCCGGACGATGTGAGCCTTGTCGGGTCAGGTGATATCGCTCCCACGAAAATTCTAACATCGGGGCAGGTAACTGACAC
CTACCTGCTTGCGCTAGCCAAGGCGCGTGGGGGCCAGCTGGCGACATTCGATCGCAAGCTGTCAGCAGCAGCGGTTACAA
GGGGCAATTCTGCCTTGCACCTGATCACCACGAGTAGATCGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10016.38 Da        Isoelectric Point: 6.2267

>AT185184 WP_001301023.1 NZ_CP066839:2611266-2611532 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Download         Length: 267 bp

>AT185184 NZ_CP088091:c722277-722050 [Rhizobium laguerreae]
ATGAGAACGACTTTGGCAATCGACGACGATGTCCTGATCGCCGCAAAAGCGATGGCAACCCAGCAGCACCGGAGCGTCGG
CGAGGTCATTTCTGAGTTGGCGAGACGTTCTCTGCGCCGACCGCCGAGCAGCGGCGAGCGCAATGGCATTCCTCTCCTGT
CAACACGGCCGGATACGCCGCCAGTGACGCTCGAAATCGTGAACGCCATGCGTGACGAACTGCCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5K98
PDB 3TPV
PDB 3DNV
PDB 3TPD
PDB 3HZI
PDB 3FBR
PDB 4YG7
PDB 3TPE
PDB 2WIU
PDB 3DNT
PDB 3TPB
PDB 3TPT
PDB 3DNU

Antitoxin

Source ID Structure

References