Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1159087..1159741 Replicon chromosome
Accession NZ_CP066839
Organism Escherichia coli strain Ec-RL2-1X

Toxin (Protein)


Gene name cptA Uniprot ID A0A140N853
Locus tag JH656_RS05715 Protein ID WP_000244783.1
Coordinates 1159334..1159741 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JH656_RS05710 Protein ID WP_000354046.1
Coordinates 1159087..1159353 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JH656_RS05685 (1154256) 1154256..1154999 + 744 WP_000951948.1 SDR family oxidoreductase -
JH656_RS05690 (1155056) 1155056..1156489 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
JH656_RS05695 (1156534) 1156534..1156845 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
JH656_RS05700 (1157009) 1157009..1157668 + 660 WP_000250274.1 hemolysin III family protein -
JH656_RS05705 (1157864) 1157864..1158844 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
JH656_RS05710 (1159087) 1159087..1159353 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JH656_RS05715 (1159334) 1159334..1159741 + 408 WP_000244783.1 protein YgfX Toxin
JH656_RS05720 (1159781) 1159781..1160302 - 522 WP_001055874.1 flavodoxin FldB -
JH656_RS05725 (1160414) 1160414..1161310 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JH656_RS05730 (1161335) 1161335..1162045 + 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JH656_RS05735 (1162051) 1162051..1163784 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15962.85 Da        Isoelectric Point: 11.2669

>T185174 WP_000244783.1 NZ_CP066839:1159334-1159741 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEISLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T185174 NZ_CP088088:1103802-1104725 [Bradyrhizobium diazoefficiens]
ATGATCGGCAAGCCGCTCAAAGATCCCGGTGCTTCGATCCGTCAGCGCCTGCTCAACCACGCCAAGGAGCACGGCGACGA
GTATCAACGGATTCTGACGCGCTACGCGATTGAGCGGTTGCTTTTCAGACTAAGCCAAACCGAGGCGCGCGAGCGATACG
TCCTCAAGGGCGCGATGCTCTTCGTGACTTGGCCCGAACAAGTGTTTCGGCCCACAGGTGATCTTGACCTTCTTGGGCAA
GGGGATCCCGATCCAGCTGCCATCATCGATCTCTTCACCCTTATCTGCCAGGTCGAGGCGCCGGCGGATGGCATCATCTT
CGACCCCTCTACGCTGCAGGTGGAGGCGGTCCGCGAGGAGGAAAAATATCAGGGTGTCAGACTCTCCCTGAGGGGACGTC
TTGGCACGGCGGTGATCCCTGTCCAGGTGGACATCGGGTTTGGGGACAGTGTTCATCCCGCGCCCAGCCGGAACAATTTC
CCCAGCCTGCTGCCGGACCTGCCGGCGGCGGACGTCCTGATGTACCCGCCCGAGACAGTAATTGCAGAGAAATTCGAAGC
GATGATCCGCTTTGGCGAAGCGAACGGCAGGATCAAAGATTTTCACGATATCTGGGTCACGGCGCGAACCTTCGCTTTCA
ACCTCTCTACGCTGGTCGAGGCGGTTAGCGGTACCCTGCAAAGGCGGGAAACCGCGTTCCCGACGGAGATGCCGGTGGCG
CTGACGTCTCAGTTCGCCGAGATGGCCGACAAACAAGCGCTATGGACAGGCTTTCTGCGCCGCAATCCGCCCACGCTGCC
GCCGCCTCCATTGGGCGACTTGTTGGCGGAGCTGCGGCGGTTCTTCGAACCCGTGCTTGCGGCTCTTCCTTTGCCGGAAA
GCGCGACCGGTCGTTGGAATCCGGACCGCGGCGCCTGGGAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT185174 WP_000354046.1 NZ_CP066839:1159087-1159353 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT185174 NZ_CP088088:1103113-1103805 [Bradyrhizobium diazoefficiens]
ATGACATCAGCTGCTAATCTTCAAATGTTGTTCAATGTCGACAAATCTGCTGACGTGCCGACAAAAGATAACAAAATGAC
CCAACGTCATGTGCTGCCTTATCGCGAGCGCGCCCTCAATCTCGTTCGGGCTCGAGGCATTGCCCGCGCCCGCGACTTCA
AAGCCGCGGGTATCCCCCTTGTTTACCTGAAGCGACTGACAGACACAGGTGAACTCGTTCGACTTGGAAGAGGGCTCTAC
CAAGATCCGGATCGAGTCGGAGAAGATGTCGCTCATAATCTTGCGGAAGCCGCACGGGCCGTTCCCAACGGAGTCGTTAG
CCTTGCCAGTGCGCTCCGGTACCACGGGTTGACGACCCAGCTGCCGCATGCCGTCTGGCTGACCATTCCGCACAAAGCTA
GAACTCCGAAGGCCATTGGCTTCCCGTTGGAAATCGTCCGCGCCACAGGCGATGTGATGACAGCTGGCGTCGAGCATGTG
CAGGTCGAAGGCGTCGACGTCCCCATATACGGCGTAGCTAAAACCATCGCCGATTGCTTCAAGCACCGTCGTCATATCGG
GGAGGACGTCGCGATCGAGGCACTCCGCGATGCGCTCCGCCTGCGGAAGACTACAGTGAGCGATGTCATGAGGTACGCCG
AGATCGATCGTGTGGCCGAGAGAATCCGCCCCGTTCTTAAGGCGCTTCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140N853

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References