185163

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4248169..4248823 Replicon chromosome
Accession NZ_CP066836
Organism Escherichia coli strain Ec-FL1-1X

Toxin (Protein)


Gene name cptA Uniprot ID B7N7E0
Locus tag JH476_RS20445 Protein ID WP_000244769.1
Coordinates 4248416..4248823 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JH476_RS20440 Protein ID WP_000354046.1
Coordinates 4248169..4248435 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JH476_RS20420 (4244257) 4244257..4245690 - 1434 WP_001513393.1 6-phospho-beta-glucosidase BglA -
JH476_RS20425 (4245735) 4245735..4246046 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
JH476_RS20430 (4246210) 4246210..4246869 + 660 WP_000250274.1 hemolysin III family protein -
JH476_RS20435 (4246946) 4246946..4247926 - 981 WP_000886049.1 tRNA-modifying protein YgfZ -
JH476_RS20440 (4248169) 4248169..4248435 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JH476_RS20445 (4248416) 4248416..4248823 + 408 WP_000244769.1 protein YgfX Toxin
JH476_RS20450 (4248863) 4248863..4249384 - 522 WP_001055874.1 flavodoxin FldB -
JH476_RS20455 (4249496) 4249496..4250392 + 897 WP_000806652.1 site-specific tyrosine recombinase XerD -
JH476_RS20460 (4250417) 4250417..4251127 + 711 WP_000715210.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JH476_RS20465 (4251133) 4251133..4252866 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16062.03 Da        Isoelectric Point: 11.5554

>T185163 WP_000244769.1 NZ_CP066836:4248416-4248823 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGK
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T185163 NZ_CP088020:427685-428035 [Radiobacillus kanasensis]
TTGATTGTTAAAAGAGGCGAAGTTTATTTCGCCGATCTCTCTCCTGTTGTTGGTTCCGAGCAGGGGGGCGTCCGTCCAGT
ACTGGTGCTGCAAAACGATATAGGTAATCGTTTTAGTCCAACGGTTATTGTTGCAGCAATTACCGCGCAGATACAAAAAG
CGAAACTACCTACTCACGTTGAAATTGACGCGCAGAAGTATGGATTTGAACGGGATTCTGTAATTCTTTTAGAACAAATA
AGAACCATTGATAAACAAAGACTGACTGATAAGATTACACAGCTTGACCAACATATGATGGAAAACATTAACAAGGCTCT
AGAAATAAGCCTTGGATTAACTGATTTTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT185163 WP_000354046.1 NZ_CP066836:4248169-4248435 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT185163 NZ_CP088020:427399-427680 [Radiobacillus kanasensis]
ATGGTTTTGTCAGAGAGCTTGCAAGAAATTGTAGTTAGACTACCAAAAAACCTACTGAATGAGGTGGACGGCTTAATGAG
AAATGAGAACAGTGATCTCAGTGACTTCATTTGCCAGGCAACAAAATCGTATTTGCGTGAAAAGAAAAAACGCCATATTC
GGGAATCCATGCAGCGCGGCTACATGGAAATGGCCAAGATAAATCTCAATATTGCTTCAGAAGCCTTTCAGGCTGAAGAG
GAGGCCGAAACCACCCTTGAACGCTTAGTGAGCGGGGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829J3T1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QD57

References