Detailed information of TA system TA185158

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3455131..3455743
• Replicon: chromosome
• Accession: NZ_CP066836
• Organism: Escherichia coli strain Ec-FL1-1X

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: U9YXE2
• Locus tag: JH476_RS16500
• Protein ID: WP_000833473.1
• Coordinates: 3455131..3455316 (+)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IWR9
• Locus tag: JH476_RS16505
• Protein ID: WP_000499744.1
• Coordinates: 3455333..3455743 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH476_RS16490 (3451963)	3451963..3453114	+	1152	WP_000741501.1	L-threonine dehydrogenase	-
JH476_RS16495 (3453315)	3453315..3454853	+	1539	WP_001309845.1	aldehyde dehydrogenase AldB	-
JH476_RS16500 (3455131)	3455131..3455316	+	186	WP_000833473.1	type II toxin-antitoxin system HicA family toxin	Toxin
JH476_RS16505 (3455333)	3455333..3455743	+	411	WP_000499744.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
JH476_RS16510 (3455815)	3455815..3457779	-	1965	WP_001026861.1	glycoside hydrolase family 127 protein	-
JH476_RS16515 (3457790)	3457790..3459190	-	1401	WP_000204802.1	MFS transporter	-
JH476_RS16520 (3459416)	3459416..3460231	+	816	WP_000891825.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6800.89 Da
• Isoelectric Point: 11.7053

>T185158 WP_000833473.1 NZ_CP066836:3455131-3455316 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Nucleotide

• Length: 186 bp

>T185158 NZ_CP088019:c1573924-1573505 [Pectobacterium carotovorum]
ATGACAGATATCTTGCTCAACAAAAGCAGCACTATACAAAGATGCCTACGTCGTATTCGTGAGGAATTCGATACTGAGGA
AGGGTTTCGTCAAAACTTCACCAAGCAAGATTCCGTCATACTTAACATACAGCGTGCCTGCGAAGCCGCTATTGATATCG
CTAATTATCTTATCCGTATCAAGCAGCTAGGCATACCACAAAGTAGCCGCGATAGCTTCGCGCTTCTTGCTGCAAACCAG
ATTATTACGGTTGATCTGTCTGATAATTTGCAAAAAATGGTGGGATTAAGAAATATTGCCGTGCATGACTATCAGGCGCT
CAATCTGGATATTGTCATCCACGCCATTTCTCATCGTTTAAGCGACTTTGAACATTTTATCAAACAAATAGCCCGGTACA
GCGACAGCCAGCACCTTTAG

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15241.15 Da
• Isoelectric Point: 4.5486

>AT185158 WP_000499744.1 NZ_CP066836:3455333-3455743 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Nucleotide

• Length: 411 bp

>AT185158 NZ_CP088019:c1574304-1573921 [Pectobacterium carotovorum]
ATGTTCGATAAGATCGTTTTGACACTGAAAAAACAGATGCCCGACATCAAAATTATTTATCTCTTTGGTTCCCAGGCTAC
AGGCAATGCCAGAGCGGACAGCGACATTGATATCGCCATCATGGCTACGAGAGCACTGGATCCCATTGAACGCTGGGAGC
TATCTAATCAATTAGCAAAAGAAGTGGGTCATGACGTCGATCTTATCGATTTATTGCAGGCTTCAACGGTATTAAAAATG
GAGATCGTTCGTAACGGCAAGCTGCTTTATGATGCGGAAACAGCAGCGGGAGAATTTGAAATGACAACACTTTCAATGTA
CCAGCATCTGCAAAAAGAGCGTGCGGATATCATTCGTAGCTTTAATCAGGATCTAAAAGCATGA

Structures

Toxin

• AlphaFold DB: U9YXE2

Antitoxin

• AlphaFold DB: L4IWR9