185157

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2834819..2835316 Replicon chromosome
Accession NZ_CP066836
Organism Escherichia coli strain Ec-FL1-1X

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JH476_RS13725 Protein ID WP_001173343.1
Coordinates 2834819..2834992 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JH476_RS13730 Protein ID WP_000398619.1
Coordinates 2835020..2835316 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JH476_RS13715 (2831490) 2831490..2832947 + 1458 WP_000856848.1 dipeptide/tripeptide permease DtpC -
JH476_RS13720 (2833184) 2833184..2834701 + 1518 WP_000003806.1 lysine--tRNA ligase -
JH476_RS13725 (2834819) 2834819..2834992 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JH476_RS13730 (2835020) 2835020..2835316 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JH476_RS13735 (2835545) 2835545..2835817 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
JH476_RS13740 (2835829) 2835829..2836059 - 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
JH476_RS13745 (2836240) 2836240..2837871 + 1632 WP_001216477.1 sensor histidine kinase -
JH476_RS13750 (2837868) 2837868..2838587 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island tet(B) / sul2 papB / papI 2781593..2835316 53723


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T185157 WP_001173343.1 NZ_CP066836:c2834992-2834819 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T185157 NZ_CP088019:1351654-1351836 [Pectobacterium carotovorum]
ATGAAATCAACTGACCTGATAAAGGAGCTGGCAGCGGCAGGTTGTGAGTGCAAACGGCATAACGGCGGGAGTCACCAAAT
ATGGTGGTCACCCATTACTGGAAAAACATTTCCAGTGCCGCATCCAAAAAAGGATTTACCACTTGGAACTGTCAAATCCA
TCAAAAAAATGGCGGGGATTTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT185157 WP_000398619.1 NZ_CP066836:c2835316-2835020 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT185157 NZ_CP088019:1351859-1352290 [Pectobacterium carotovorum]
ATGTTTTTTTCAGTCGGTGTTGAAACGCCAAAAGATGATGCTACGGCATATGGGCTGATCGTTCCGGCGTTATGTACTGA
TGATTATGGCTGCTTCTCTGCAGCTGACAGCCAACAAGAGATTGCACGCATGGCGCGGGAAGCGATTTTAACCCTGATTG
ATGAAGCGGTGAGCCGCGGAAATCTGGATATTAAACGACTTAATGACGCTGGACATCTCATCTATGCGGCAATGCCAGAT
TTTGCGGACTACGATAGTTGGTTCGTGCTGGATGTGGATTTATCGGTTTTTGAGGGAAAACCGCAGCGATTGAACATTTC
ACTTCCCGATACGTTGATACAGCGTATTGATAATCGCGTGCGAGAGCAGCCGGGTACTTATCGCGATCGCAGCCATTTTT
TGGCAGAAGCCGCACGCCATGAACTGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References