185152

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1882729..1883347 Replicon chromosome
Accession NZ_CP066836
Organism Escherichia coli strain Ec-FL1-1X

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JH476_RS09230 Protein ID WP_001291435.1
Coordinates 1883129..1883347 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JH476_RS09225 Protein ID WP_000344800.1
Coordinates 1882729..1883103 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JH476_RS09215 (1877818) 1877818..1879011 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JH476_RS09220 (1879034) 1879034..1882183 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
JH476_RS09225 (1882729) 1882729..1883103 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JH476_RS09230 (1883129) 1883129..1883347 + 219 WP_001291435.1 HHA domain-containing protein Toxin
JH476_RS09235 (1883519) 1883519..1884070 + 552 WP_000102559.1 maltose O-acetyltransferase -
JH476_RS09240 (1884186) 1884186..1884656 + 471 WP_000136192.1 YlaC family protein -
JH476_RS09245 (1884820) 1884820..1886370 + 1551 WP_001389864.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JH476_RS09250 (1886412) 1886412..1886765 - 354 WP_000878140.1 DUF1428 domain-containing protein -
JH476_RS09260 (1887144) 1887144..1887455 + 312 WP_000409911.1 MGMT family protein -
JH476_RS09265 (1887486) 1887486..1888058 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T185152 WP_001291435.1 NZ_CP066836:1883129-1883347 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T185152 NZ_CP088019:c507442-507188 [Pectobacterium carotovorum]
GTGAAGCTAATCTGGTCAGAAGAAGCATGGGAAGACTACCTGTACTGGCAGGACATCGATAAGCGGATGGTCAAAAAGAT
CAATGAATTAATAAAAGAAACGCGCAGAACACCTTTTGAAGGAAAAGGAAAACCAGAGCCACTTAAACATAACCTTGCTG
GTTTCTGGTCGCGCCGCATCACCGAAGAACACCGTCTGGTTTATGCCATCACCGATGACGCCATGATGATCGCTGCCTGC
CGCTACCACTATTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT185152 WP_000344800.1 NZ_CP066836:1882729-1883103 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT185152 NZ_CP088019:c507690-507439 [Pectobacterium carotovorum]
ATGCGTACAATTAGCTACAGTGAAGCCCGACAAAATCTATCGGCGACCATGATGAAAACGGTCGAAGACCGTGCTCCCAT
TCTTATCACCCGACAGAATGGTGAAGCCTGCGTGCTCATGTCTTTGGAAGAGTATAACTCTCTAGAGGAGACCGCGTATT
TACTGCGTTCACCAGCCAATGCAAAAAGACTGATGAACTCAATCGAGAGCCTTAAGGCTGGCAATGGCAAAGAAAGAGAT
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References