Detailed information of TA system TA185151

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1434248..1434953
• Replicon: chromosome
• Accession: NZ_CP066836
• Organism: Escherichia coli strain Ec-FL1-1X

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: JH476_RS07140
• Protein ID: WP_000539521.1
• Coordinates: 1434248..1434634 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JH476_RS07145
• Protein ID: WP_001280945.1
• Coordinates: 1434624..1434953 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH476_RS07120 (1430252)	1430252..1430878	+	627	WP_001295292.1	glutathione S-transferase GstB	-
JH476_RS07125 (1430875)	1430875..1431990	-	1116	WP_000555038.1	aldose sugar dehydrogenase YliI	-
JH476_RS07130 (1432101)	1432101..1432484	-	384	WP_000497137.1	biofilm formation regulator BssR	-
JH476_RS07135 (1432697)	1432697..1434022	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
JH476_RS07140 (1434248)	1434248..1434634	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JH476_RS07145 (1434624)	1434624..1434953	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
JH476_RS07150 (1435023)	1435023..1436351	-	1329	WP_000086879.1	GGDEF domain-containing protein	-
JH476_RS07155 (1436359)	1436359..1438707	-	2349	WP_001322378.1	EAL domain-containing protein	-
JH476_RS07160 (1438885)	1438885..1439796	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T185151 WP_000539521.1 NZ_CP066836:1434248-1434634 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T185151 NZ_CP088019:489357-490655 [Pectobacterium carotovorum]
ATGAATATTCTCACGGTGGCGCTTAATGGCATTGCCGTCGGGGCGCTAGCGAAGGGGGTTGGGAGCGATATGACGTTCAC
TTACTACCCTGATTGGCTGAGCCGTCCCGGTGCGCGCGCTATTTCTCTTTCATTGCCTCTACAGCGGGCCACCTATCGTG
GCGATGTGGTTTACAACTTCTTTGATAATTTATTACCTGACAACGACAGTATTCGCAGCAGGATCCAGGCTCGCTTTCGT
ATCGCGACGAAACAGCCCTTTGATTTACTGACTAGCATCGGCGGTGACTGCGTTGGTGCGGTTCAGATCTATCCGGTCGA
TACACCTGTTGCCCCTGTCACTGATGTACATGCAGAAGCGCTTGATAATGATGAGATTGAAGCGTTGCTGCAGGGTTATC
GGGAAGCGCCATTAGGGATGGCGATGGATCGTGAGGATTTTCGTATCTCTCTCGCCGGTGCGCAGGAAAAGACGGCGCTA
CTTTGGTATAAAAATCAGTGGCATCGTCCGCATGGGAGTACGCCGACCAGCCATATTTTCAAATTACCGATTGGTTATAT
CGCCAACAACAGCATTGACCTCAGCGACAGTGTTGAAAACGAGTGGTTATGCCTGAAAATCGCCGAAAAGTATGGCTTTA
CGGTCGCTCAGGCGGAAATTTCCCGATTTAACTCACAAAAAGTCTTGATCGTTGAACGCTTTGATCGTCGCTGGTCACGC
AGCGATAAGCCCTGGCTGATGCGTTTGCCACAGGAGGATTTTTGTCAGGCACTGGGTATTGCGCCTGCGTTAAAATACGA
GTCTGACGGTGGCCCTGGCATTGAGAAATCAATGGTGCTCTTGCTCGGTTCGCAACAGGCGATGCAGGATAGAGATACCT
TTTTCAAAAGCCAGATACTGTTTTGGATGCTAGCCGCGATTGATGGTCATGCGAAAAACTTCAGTGTATTTATCGAGCCA
GGTAGTGCATACCGTATGACGCCTTTATACGATGTGATATCCGCTTTTCCTTTCATGGGACCGGCAGGAATATCGGTCCA
GAAGGCGAAGATGGCGATGGCGGTTCTTGGGCGTAATCGTCATTTCCATTGGCATAAGATTTTGCCACGGCACTATTTTT
CTAGCGCCAGACAAGTGGGCTATCCGCAGGAACGCGTTGCCGCAATGATGTCGGAGATGAAAGCAAATACGGAACGGGTC
ATTGCGGAGGTGGAGGCACTCCTTCCCGACGATTTTCCTGAACGTATCAGTCATCCTATTTTTGCGGGACTGCGATCGCA
GGCTGCTCGGCTTCCCTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT185151 WP_001280945.1 NZ_CP066836:1434624-1434953 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT185151 NZ_CP088019:489105-489353 [Pectobacterium carotovorum]
ATGAAGATCACCTCTGCTGGCATGCTAGCCCATGCTATGCGCGATGAACGCAAAAAGCACAAACTGAATCAAAGCCAAGT
TGCCGAGAGTATGGGAATAAAGCAGTCCACGGTGTCTGATTTCGAGAATCACCCAGAAGGGACGCGGCTTGAAACATTGT
TCAAGCTGCTGGCGGCTCTGGATCTTGAACTTCATCTTTCCAAACGAGGGGAGCCACAAGCGGGTCAGGAATGGAGTGAG
GAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6