Detailed information of TA system TA185150

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1304394..1305234
• Replicon: chromosome
• Accession: NZ_CP066836
• Organism: Escherichia coli strain Ec-FL1-1X

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: JH476_RS06505
• Protein ID: WP_000854686.1
• Coordinates: 1304394..1304777 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0J1S3
• Locus tag: JH476_RS06510
• Protein ID: WP_001285602.1
• Coordinates: 1304854..1305234 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH476_RS06475 (1301070)	1301070..1301774	+	705	WP_001241678.1	leucyl/phenylalanyl-tRNA--protein transferase	-
JH476_RS06480 (1302059)	1302059..1302277	+	219	WP_001040187.1	translation initiation factor IF-1	-
JH476_RS06490 (1302768)	1302768..1303610	-	843	WP_001274547.1	DUF4942 domain-containing protein	-
JH476_RS06495 (1303695)	1303695..1303892	-	198	WP_000839253.1	DUF957 domain-containing protein	-
JH476_RS06500 (1303909)	1303909..1304397	-	489	WP_001054233.1	DUF5983 family protein	-
JH476_RS06505 (1304394)	1304394..1304777	-	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
JH476_RS06510 (1304854)	1304854..1305234	-	381	WP_001285602.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JH476_RS06515 (1305245)	1305245..1305928	-	684	WP_000086769.1	hypothetical protein	-
JH476_RS06520 (1305947)	1305947..1306168	-	222	WP_000692298.1	DUF987 domain-containing protein	-
JH476_RS06525 (1306231)	1306231..1306707	-	477	WP_001186726.1	RadC family protein	-
JH476_RS06530 (1306723)	1306723..1307208	-	486	WP_000214307.1	antirestriction protein	-
JH476_RS06535 (1307300)	1307300..1308118	-	819	WP_001234732.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T185150 WP_000854686.1 NZ_CP066836:c1304777-1304394 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T185150 NZ_CP088019:c429965-429603 [Pectobacterium carotovorum]
ATGAGAGTCATCTCAGCCAAGGCCATCACCGATGCGATGAAAAAGCATGCGAAATGGAAAGTTGGTTTGAAGTTATGGCT
GGATGTTTTTGACAAGCCATCGCTGCGGTTTGAGTCCTATGAACAGTTATGTCATGTATGGCGAAATACATCTTGCTGGA
ATGTCGATCGCATACCTTTCGCATTGCTAGAAAACGAAAGCCGCAAAGGACCACTTGATATATATATATTTGATATTAAT
GGTAACAATTGCAGAATCATAGCATGGTTAAACCCTAATACAGGTTCTTTGTATGTAAAAGACGTGTGCTCTCACGCAGA
GTACGATAAGTGGTGGAAAGCTCAAACGAAGACAAAAAGGTGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13938.69 Da
• Isoelectric Point: 5.0823

>AT185150 WP_001285602.1 NZ_CP066836:c1305234-1304854 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT185150 NZ_CP088019:c429599-429126 [Pectobacterium carotovorum]
ATGCGAAATACATCTCTGGGTTCTAACAGTTCTGTAGGAATCAGTGGGCTCATCGAAAATTTACCTCTTCTACTTCAGGA
AGCCTCTGAGCAGCTTAACAGCCTGTCTGTTTTACTCTCTGCCTGTATGGAACCTATTGAAGACGATAATGATCTTCAGG
AAAGAATGGAGTGCATCAATCAACTTTACCTCTATTCCTCTGATGTCAATGATATTCCAGCCACTTTTGCAGAGCTCATC
GCGGACAGAGTCTATGAATATGAAAAGAAACACCGAGATGTGCCCCACGTCAGTCAGGCTGAAGCCTTAGCTTTTTTTAT
TGAAGAAAGAGGGCTGAAACAGTCAGATTTAAAGCAAATTGCAACGCAAAGCGTCATCTCTGACATTATTAACGGTAAGC
GAACCATGACATTACAGCAAGTTAAGGATTTTTCTCGCTACTTTAATGTGCCTGTTGAGACATTTATTGACTGA