Detailed information of TA system TA185149

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1110895..1111679
• Replicon: chromosome
• Accession: NZ_CP066836
• Organism: Escherichia coli strain Ec-FL1-1X

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: JH476_RS05590
• Protein ID: WP_000613626.1
• Coordinates: 1111185..1111679 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: JH476_RS05585
• Protein ID: WP_001110447.1
• Coordinates: 1110895..1111188 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH476_RS05575 (1106045)	1106045..1107004	-	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
JH476_RS05580 (1107577)	1107577..1110762	+	3186	WP_000827427.1	ribonuclease E	-
JH476_RS05585 (1110895)	1110895..1111188	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
JH476_RS05590 (1111185)	1111185..1111679	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
JH476_RS05595 (1111774)	1111774..1112727	-	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
JH476_RS05600 (1112739)	1112739..1114382	-	1644	WP_000096524.1	flagellar hook-associated protein FlgK	-
JH476_RS05605 (1114448)	1114448..1115389	-	942	WP_001309406.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
JH476_RS05610 (1115389)	1115389..1116486	-	1098	WP_000589320.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T185149 WP_000613626.1 NZ_CP066836:1111185-1111679 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T185149 NZ_CP088019:377849-378136 [Pectobacterium carotovorum]
ATGACCTATAAGTTAAAATTTGTGCCTTCTGCAATGAAGGAATGGAAAAAATTAGGGCATCCTGTCCGGGAGCAGTTCAA
GAAAAAGCTGGTTGAGCGTTTGGAAAATCCTCGTGTTCCTTCAGCCCAACTTCATGGCCGTAAAGACCAGTATAAAATTA
AACTGAAGTCCGCTGGCTACAGGCTGGTGTATTTGGTCCAGGATGAGACCATTACTGTGATGGTTATGGGCGTTGGAAAG
CGCGAAGGTAGCCAGATTTATTCTGATACTAAAGGTCGCTCTGCTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT185149 WP_001110447.1 NZ_CP066836:1110895-1111188 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT185149 NZ_CP088019:377605-377859 [Pectobacterium carotovorum]
ATGGCTTTCAACATCCTGACTAACACAGCCGCCAGTATTACTGAGTTGAAACGTGACCCGATGGGGACGGTACGGGCTGC
TGAAGGTGAAACTATCGCTATACTGAACCGGAATGAGCCGGCGTTCTACTGTGTGCCTCCTGCCGTATTCGCTTATTTAA
TGGAACTGGCTGAAGATGCCGAACTGGGGCGTACTGTTGATGATCGGATGTCAGAGTTAGCTGACGCGGAAGAGATTTCG
CTCGATGACCTATAA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6