Detailed information of TA system TA185148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 767275..767913
• Replicon: chromosome
• Accession: NZ_CP066836
• Organism: Escherichia coli strain Ec-FL1-1X

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B7N4J4
• Locus tag: JH476_RS03790
• Protein ID: WP_000813797.1
• Coordinates: 767737..767913 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JH476_RS03785
• Protein ID: WP_076611057.1
• Coordinates: 767275..767691 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH476_RS03765 (762427)	762427..763368	-	942	WP_001251335.1	ABC transporter permease	-
JH476_RS03770 (763369)	763369..764382	-	1014	WP_000220413.1	ABC transporter ATP-binding protein	-
JH476_RS03775 (764400)	764400..765545	-	1146	WP_000047447.1	ABC transporter substrate-binding protein	-
JH476_RS03780 (765790)	765790..767196	-	1407	WP_000760613.1	PLP-dependent aminotransferase family protein	-
JH476_RS03785 (767275)	767275..767691	-	417	WP_076611057.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JH476_RS03790 (767737)	767737..767913	-	177	WP_000813797.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JH476_RS03795 (768135)	768135..768365	+	231	WP_000494244.1	YncJ family protein	-
JH476_RS03800 (768457)	768457..770418	-	1962	WP_001309488.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JH476_RS03805 (770491)	770491..771027	-	537	WP_000429161.1	DNA-binding transcriptional regulator SutR	-
JH476_RS03810 (771119)	771119..772291	+	1173	WP_001236223.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6724.76 Da
• Isoelectric Point: 11.5666

>T185148 WP_000813797.1 NZ_CP066836:c767913-767737 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T185148 NZ_CP088019:c213567-213388 [Pectobacterium carotovorum]
ATGGATAGCAGGACGTTAATAGCAGAAATCAAGGCCGATGGGTGGGAACTGATAAGAGTGAACGGAAGCCACCACCATTT
TATGCACCCTAGCAAACCGGGGTTAGTCACCATCCCCCACCCTAAGAAAGATTTACCGATAGGCACGGTAAAAAGCATCA
GGAAACAGGCAGGAATATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15251.65 Da
• Isoelectric Point: 4.5908

>AT185148 WP_076611057.1 NZ_CP066836:c767691-767275 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT185148 NZ_CP088019:c213360-212950 [Pectobacterium carotovorum]
ATGTTTTATCCGATTGCAATTGAAGCAGGCGACGATACCCACGCATACGGTGTAACCGTGCCAGATCTACCGGGCTGTTT
TTCTGCAGGCGATACGCTAGACGATGCCATCGCGAATGCTAAAGAGGCGATTACCGGACATATCGAACTGCTTATCGAGA
TGGGTCAGGATATTCCCACCGTGTCGTCAGTAGGCCAACTGGCAAAAAACGCAGAATACGCTGGGTACACGTGGGCGGTT
GTTGATATCGATGTGACGCGTTTAATGGGCGGTTCAGAAAAAATTAACGTTACACTGCCTAAGTCGCTGATTGACCGCAT
TGATCGTTGCGTGGCAAGTAATCCTGAATTTAAAAGCCGCTCAGGGTTTCTCGCGCAGGCAGCATTAGAGCGAATCTCGT
CTTCACGATGA

Structures

Toxin

• AlphaFold DB: A0A829J715