Detailed information of TA system TA185146

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 667540..669128
• Replicon: chromosome
• Accession: NZ_CP066836
• Organism: Escherichia coli strain Ec-FL1-1X

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: JH476_RS03345
• Protein ID: WP_001546825.1
• Coordinates: 667806..669128 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: JH476_RS03340
• Protein ID: WP_001296726.1
• Coordinates: 667540..667806 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH476_RS03340 (667540)	667540..667806	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
JH476_RS03345 (667806)	667806..669128	+	1323	WP_001546825.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
JH476_RS03350 (669526)	669526..670425	-	900	WP_001057776.1	LysR family transcriptional regulator	-
JH476_RS03355 (670565)	670565..670873	+	309	WP_001221046.1	hypothetical protein	-
JH476_RS03360 (670882)	670882..671571	+	690	WP_000801313.1	type 1 glutamine amidotransferase domain-containing protein	-
JH476_RS03365 (671645)	671645..672592	+	948	WP_000854796.1	aldo/keto reductase	-
JH476_RS03370 (672695)	672695..672850	+	156	WP_001322415.1	hypothetical protein	-
JH476_RS03375 (672810)	672810..673229	+	420	WP_001322416.1	amidohydrolase family protein	-
JH476_RS03380 (673219)	673219..673371	+	153	WP_001322417.1	amidohydrolase family protein	-
JH476_RS03385 (673522)	673522..674094	+	573	WP_000789913.1	NAD(P)H-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49331.00 Da
• Isoelectric Point: 8.1369

>T185146 WP_001546825.1 NZ_CP066836:667806-669128 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHCRLSREYGIK

Nucleotide

• Length: 1323 bp

>T185146 NZ_CP088018:3462022-3462426 [Ruania suaedae]
GTGAGCACGCTCTACCTGCTCGACACCAACGTGCTCGTCGCCCTCCTGCGTGGCCAGGCTGAGCGTGCGCACCCCCGGCT
GCGTGCCGCCGAGGGCCGGATCGCCGTCTCCACGATCAGCGAGATGCAGCTGGAGTACGGCGTCGAGCGTTCACAGGAGC
CGCTGCGGAATCGGCAAGCGGTTGACGAACTGCTCTCGCTCGTCGAGATCCTGGAGTTCGACACGATCGCGGCCATGCAC
GCCGGACGGATCCGTGCGGTCCTGGCGTCTGCCGGGGCTCCGATCGGTCCGTTCGACAGCCTGCTGGCCGGACATGCGCG
CTCACTCGGGCTGGTGCTCGTCACGAACAACACCCGGGAGTTCACTCGCGTGCCCGGCCTGGAGGTCGAGGACTGGCTGG
CCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT185146 WP_001296726.1 NZ_CP066836:667540-667806 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT185146 NZ_CP088018:3461795-3462025 [Ruania suaedae]
ATGCGGAGCACGGTGTTCAGAAACAACCGGACGCAGGCCGTCCGCCTGCCGAAGGCTGCTGCACTTCCGGAGGACGTCCG
CGAGGTGAATGTGCGCGTGATCGGGCGCAGCCGCGTGATCGAGCCCGTGGGGTCCAGTTGGGACGAGTGGTTCGAGCACG
GCCTGCGCACGAGCGCCGACTTCCTCGCCGACCGCGACCAGGGCACTGCAGAGGAGCGGCCCGGGTTGTGA