Detailed information of TA system TA185134

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-MqsA
• Location: 3649445..3650144
• Replicon: chromosome
• Accession: NZ_CP066833
• Organism: Proteus mirabilis strain RGF134-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: JEQ15_RS16880
• Protein ID: WP_017628658.1
• Coordinates: 3649445..3649831 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B4EZ97
• Locus tag: JEQ15_RS16885
• Protein ID: WP_004246828.1
• Coordinates: 3649824..3650144 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JEQ15_RS16865 (JEQ15_16830)	3645464..3646045	-	582	WP_012368524.1	DNA-3-methyladenine glycosylase I	-
JEQ15_RS16870 (JEQ15_16835)	3646296..3647201	+	906	WP_004246832.1	glycine--tRNA ligase subunit alpha	-
JEQ15_RS16875 (JEQ15_16840)	3647211..3649283	+	2073	WP_017628659.1	glycine--tRNA ligase subunit beta	-
JEQ15_RS16880 (JEQ15_16845)	3649445..3649831	+	387	WP_017628658.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JEQ15_RS16885 (JEQ15_16850)	3649824..3650144	+	321	WP_004246828.1	helix-turn-helix domain-containing protein	Antitoxin
JEQ15_RS16890 (JEQ15_16855)	3650193..3650627	-	435	WP_004246827.1	hypothetical protein	-
JEQ15_RS16895 (JEQ15_16860)	3650620..3651504	-	885	WP_012368526.1	endonuclease/exonuclease/phosphatase family protein	-
JEQ15_RS16900 (JEQ15_16865)	3651719..3653005	-	1287	WP_063073863.1	DUF3748 domain-containing protein	-
JEQ15_RS16905 (JEQ15_16870)	3653142..3653759	+	618	WP_004246822.1	trimeric intracellular cation channel family protein	-
JEQ15_RS16910 (JEQ15_16875)	3654060..3654683	+	624	WP_004246821.1	guanylate kinase	-
JEQ15_RS16915 (JEQ15_16880)	3654738..3655013	+	276	WP_004246820.1	DNA-directed RNA polymerase subunit omega	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-125)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-95)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14322.65 Da
• Isoelectric Point: 10.0826

>T185134 WP_017628658.1 NZ_CP066833:3649445-3649831 [Proteus mirabilis]
MVIYKTKMFKNSFKKITLTNAELIAIATEVLAGQFEADLGGGVIKKRACIQGKGKSSGIRTIIFYKQGNNLFFADGWKKS
SLSSKKTKEITDDELESYKDLAKDLFNANQNKIDKMIALGLLTEVRYD

Nucleotide

• Length: 387 bp

>T185134 NZ_CP088008:c3423434-3423138 [Tardiphaga sp. 37S4]
ATGATCGTCGGGTTTCGCGATGATTGGTTGCGGGCCTTCTTCGTGGACGACGTCCATTCTCGCAACATTCCGGCAGACCT
TGAAAGCCGACTGTTTCGCAAGCTTCAGATGATCGACGATGCGACCACCGATCAGGATCTGCGCGTACCGCCGAGCAATC
ATTTCGAAAAGCTGCGCGGCAAACTTGGCGGCTCGCACTCGATCCGCGTCAACAACCAATGGCGGCTGATCTTCCGCTGG
GATAGCAGCCGCGGCGAAGCGTCGGATCTTTATCTCGACGATCACAGCTATCGATGA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12126.21 Da
• Isoelectric Point: 10.4411

>AT185134 WP_004246828.1 NZ_CP066833:3649824-3650144 [Proteus mirabilis]
MTDKMKEMLKFAQEMEDAGVTDGSTTKRIAARIKAKELQAKYKHPTPMTGEDIKRIREQNNISQSSLALILNMTKESVSK
WERNEIKPNGAALRLLHLIDKKILFI

Nucleotide

• Length: 321 bp

>AT185134 NZ_CP088008:c3423129-3422821 [Tardiphaga sp. 37S4]
ATGCTGATGACCAAACGCAAGCCGGCCACTGTCGGTGAAATCCTGGTCGAGGAATTCATGCAGCCGCTTGGCCTGACACA
AGGGACATTGGCAAAGGCGATGGGTGTCCAGCGCAAGCATGTGAACGAGTTGTGCAACGATCGCCGTAACGTGACCGCGG
CGACCGCGCTCATTCTCGCGCGCGTCTTCGGCAACAGCCCGGACTTCTGGCTGAACGTGCAGCGCCGCGGCGATTTGTGG
GAGGCGATGAACTCGCCGCAGGAACTAGCGCGGATCGAACGTGCGACGCCTCTGGTGAAGGCGGCTTAG

Structures

Antitoxin

• AlphaFold DB: B4EZ97