Detailed information of TA system TA185131

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3111848..3112869
• Replicon: chromosome
• Accession: NZ_CP066833
• Organism: Proteus mirabilis strain RGF134-1

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: JEQ15_RS14520
• Protein ID: WP_004249488.1
• Coordinates: 3112300..3112869 (+)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: B4F2H3
• Locus tag: JEQ15_RS14515
• Protein ID: WP_004245528.1
• Coordinates: 3111848..3112300 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JEQ15_RS14495 (JEQ15_14445)	3107634..3108164	-	531	WP_004245524.1	RNA pyrophosphohydrolase	-
JEQ15_RS14500 (JEQ15_14450)	3108481..3109446	+	966	WP_049195511.1	thiamine ABC transporter substrate binding subunit	-
JEQ15_RS14505 (JEQ15_14455)	3109446..3111026	+	1581	WP_004245526.1	thiamine/thiamine pyrophosphate ABC transporter permease ThiP	-
JEQ15_RS14510 (JEQ15_14460)	3111019..3111720	+	702	WP_004245527.1	thiamine ABC transporter ATP-binding protein ThiQ	-
JEQ15_RS14515 (JEQ15_14465)	3111848..3112300	+	453	WP_004245528.1	helix-turn-helix domain-containing protein	Antitoxin
JEQ15_RS14520 (JEQ15_14470)	3112300..3112869	+	570	WP_004249488.1	PIN domain-containing protein	Toxin
JEQ15_RS14525 (JEQ15_14475)	3112985..3115339	+	2355	WP_017628571.1	DNA polymerase II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• SinI-like, DNA-binding domain (80-125)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21600.10 Da
• Isoelectric Point: 7.3470

>T185131 WP_004249488.1 NZ_CP066833:3112300-3112869 [Proteus mirabilis]
MTNYTVLLDACVMYPAPLRSYLMYLANTGLFRARWSNQIHDEWITNLLVNNPTLTAERLIRTRDLMNAHVPDCLVEGYEP
LICGLKLPDPNDRHVLAAAIKGQAQAIITFNLKDFPQKYLNSFDISAIHPDEFLSDMFELNPSSCIKAAQQHRQSLKNPP
MTVETFLGCLQKQKLPQFISHLRAFQILL

Nucleotide

• Length: 570 bp

>T185131 NZ_CP088007:c109879-109607 [Nocardia huaxiensis]
ATGGCGGACCAGGAGTGGGATATCTACATCGTCGACGAGGTAAGGCAATGGATCGAGCGTCTCGACGAGCCGACCTTCCG
CCGCGTAGTGGAAGCCATCGACGCCCTCGCCGATGGCGGACCGGGGTTGGGTCGTCCGCTGGTCGACTCAATTGCCGGTT
CCAAGGTCTCGAATATGAAAGAGCTCCGTCCCGGAACCGTACGGATCCTTTTCGCGTTCGATCCGTGGCGGTCGAGCGTA
CTGCTCGCGGCGGGCGACAAAGTCCACCAATAG

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16700.15 Da
• Isoelectric Point: 5.7723

>AT185131 WP_004245528.1 NZ_CP066833:3111848-3112300 [Proteus mirabilis]
MNTISERLSAEDIALAKLSSQELSAVMDINGKTQKLSIIDKTGNNHIVQIPNSALRLMIEILTQLGQGNTVSITPIHAEL
TTQEAADILNMSRPTFVKLLDTRAIPYSRKGNRRKVAYADIVAYKNELEQKRLDALAELSQLDQEMDMGY

Nucleotide

• Length: 453 bp

>AT185131 NZ_CP088007:c109517-109200 [Nocardia huaxiensis]
ATGAGCGGACATGTGCGTTGGCAAGACATTCGAGCCGAAATGGTGGATCGGGCAGGTGGCGAAGCTGCGGTTGCCAAGGG
GAAGGACGAACTGCTCGCTCAGGTTCGCGGACAGCGGCTCGCCGATATCCGGCGCAGACGTGGGTTGACTCAACTGCAGG
TCGCCGACCGGATGGGTGTCACGAAGGGGCGAGTTTCGCAGATCGAGCAAGGCACTGTCACCGGCCAGGAAGTTCTCGCA
CGGTATGCGCTGGCATTGGGCGGCCGACTCCATCAGTCGATCTACTTCGATGACGGGGATATCGTCGCTATCGCCTGA

Structures

Antitoxin

• AlphaFold DB: B4F2H3