Detailed information of TA system TA185130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1725106..1725905
• Replicon: chromosome
• Accession: NZ_CP066833
• Organism: Proteus mirabilis strain RGF134-1

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: A0A8E3RD10
• Locus tag: JEQ15_RS08255
• Protein ID: WP_004247966.1
• Coordinates: 1725381..1725905 (+)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: B4EVS2
• Locus tag: JEQ15_RS08250
• Protein ID: WP_004247964.1
• Coordinates: 1725106..1725384 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JEQ15_RS08225 (JEQ15_08195)	1720456..1721538	+	1083	WP_004242680.1	peptide chain release factor 1	-
JEQ15_RS08230 (JEQ15_08200)	1721538..1722386	+	849	WP_004247962.1	peptide chain release factor N(5)-glutamine methyltransferase	-
JEQ15_RS08235 (JEQ15_08205)	1722364..1723179	+	816	WP_004242688.1	invasion regulator SirB1	-
JEQ15_RS08240 (JEQ15_08210)	1723237..1724091	+	855	WP_004242689.1	3-deoxy-8-phosphooctulonate synthase	-
JEQ15_RS08245 (JEQ15_08215)	1724099..1725028	+	930	WP_017628160.1	TIGR01212 family radical SAM protein	-
JEQ15_RS08250 (JEQ15_08220)	1725106..1725384	+	279	WP_004247964.1	DUF1778 domain-containing protein	Antitoxin
JEQ15_RS08255 (JEQ15_08225)	1725381..1725905	+	525	WP_004247966.1	hypothetical protein	Toxin
JEQ15_RS08260 (JEQ15_08230)	1725986..1727686	-	1701	WP_017628159.1	C4-dicarboxylic acid transporter DauA	-
JEQ15_RS08270 (JEQ15_08240)	1728650..1729591	+	942	WP_004242695.1	glyoxylate/hydroxypyruvate reductase GhrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (5-87)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19192.12 Da
• Isoelectric Point: 9.3561

>T185130 WP_004247966.1 NZ_CP066833:1725381-1725905 [Proteus mirabilis]
MTIPNWHEEAISKKHNRNGFNCGDAVLNQFLYRHARQNHENGSAKTYLAINNSNNIILGYYSLCPASIEFERTPELIKHG
LARHDIPVFKLARLATDLSVQGKGLGGQLLLAAGRRCLSVASELGGVALLIDAKNERVANWYISYGAIPLLDTPLSLLIS
FKTIYTALSMANKI

Nucleotide

• Length: 525 bp

>T185130 NZ_CP088003:49370-49702 [Staphylococcus epidermidis]
ATGATAAATCAGTTTGATGTTATTATGATTGATTTAAACCCTACACGAGGAAAAGAAAAACAAAAATATCGACCATGTGT
AGTCGTAAGTAATGATTTTGTAAATCAAAATTCTCCATTTGTATGGATTTTACCTATCACTAATAGATCGAAAAGATTTC
CATCAGATATACCTATTGAAACTAAAAATAATAGCGTGACGGGTATTATTGATTCTATACAAATTAGATCTTTAGATGCA
AAAGTAAGAAATTTCAAAAAGATTGATGAGCTGGTTGATAGTCTTAAAACAGATGTGACTAATACAATTATTGCACATTC
ATGTATTGTCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10252.82 Da
• Isoelectric Point: 5.1369

>AT185130 WP_004247964.1 NZ_CP066833:1725106-1725384 [Proteus mirabilis]
MAQALVENSERVSIRITTEDKSLLVRAASLENTNLTEFVLRNIVPVARQVIENNERLKLSEKDTLRVMELLDNPPKPNAK
LLAAAFALPDNA

Nucleotide

• Length: 279 bp

>AT185130 NZ_CP088003:49128-49373 [Staphylococcus epidermidis]
ATGAGTAAACTAGCTAAAGTAGTTAAGAATGGTAATAGCCAAGCGATCACTTTGAACAAAAGTGTTTTAAATGAATCAGG
TCTAAATATTGGTGATAGTTTGTCAGTAGAGGTTAATGACGGGGAGATTAAATTCACAAAAAATAATAAATCAATCATAG
ATGAAATTCATGATTTTTATCGTAATGGTGGATATTACGATGAAGAAGAAATTGATTTTGGAAAATCAGTAGGTTGTGAA
TTATGA

Structures

Antitoxin

• AlphaFold DB: B4EVS2