Detailed information of TA system TA185129

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1485068..1485713
• Replicon: chromosome
• Accession: NZ_CP066833
• Organism: Proteus mirabilis strain RGF134-1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B4ET86
• Locus tag: JEQ15_RS07015
• Protein ID: WP_004247035.1
• Coordinates: 1485528..1485713 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: B4ET85
• Locus tag: JEQ15_RS07010
• Protein ID: WP_004247034.1
• Coordinates: 1485068..1485481 (-)
• Length: 138 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JEQ15_RS06980 (JEQ15_06980)	1480621..1480779	-	159	WP_004247025.1	hypothetical protein	-
JEQ15_RS06985 (JEQ15_06985)	1481231..1481407	+	177	WP_004247793.1	hypothetical protein	-
JEQ15_RS06990 (JEQ15_06990)	1481578..1482324	-	747	Protein_1340	methyltransferase domain-containing protein	-
JEQ15_RS06995 (JEQ15_06995)	1483174..1483752	+	579	WP_017628400.1	alpha/beta hydrolase	-
JEQ15_RS07000 (JEQ15_07000)	1483865..1484107	-	243	WP_230458803.1	hypothetical protein	-
JEQ15_RS07005 (JEQ15_07005)	1484472..1484972	-	501	WP_004247032.1	response regulator	-
JEQ15_RS07010 (JEQ15_07010)	1485068..1485481	-	414	WP_004247034.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
JEQ15_RS07015 (JEQ15_07015)	1485528..1485713	-	186	WP_004247035.1	type II toxin-antitoxin system HicA family toxin	Toxin
JEQ15_RS07020	1485819..1486286	+	468	WP_223293438.1	DUF1073 domain-containing protein	-
JEQ15_RS07025	1486229..1486597	+	369	WP_223293455.1	DUF1073 domain-containing protein	-
JEQ15_RS07030	1486603..1486824	+	222	Protein_1348	hypothetical protein	-
JEQ15_RS07035 (JEQ15_07025)	1487153..1487704	-	552	WP_017628396.1	PH domain-containing protein	-
JEQ15_RS07040 (JEQ15_07030)	1487768..1488082	-	315	WP_017628395.1	hypothetical protein	-
JEQ15_RS07050 (JEQ15_07040)	1488489..1490222	-	1734	WP_017628394.1	LPS biosynthesis-modulating metalloenzyme YejM	-
JEQ15_RS07055 (JEQ15_07045)	1490246..1490473	-	228	WP_004247042.1	YejL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1482326..1497695	15369

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 7049.30 Da
• Isoelectric Point: 10.9659

>T185129 WP_004247035.1 NZ_CP066833:c1485713-1485528 [Proteus mirabilis]
MKSSELIKLLEKNGWILDRIKGSHHQFTHPDFSFVVTVPHPRKDLKKGTLNQIIKSAKLKN

Nucleotide

• Length: 186 bp

>T185129 NZ_CP088002:c1658816-1658607 [Staphylococcus epidermidis]
ATGAAATTGAAAAAAGGATTGATAAATGTTTCAGGTATTAAAACAACTGAACAAGCTAAACAGCTCAAAGATCATTTATC
TAAGATGATAGGCATCAATAGTGTGGATATCGATTACCAAATGAATGAGATTCGAGTAGAATTTGACACTCCTGCAAATT
TAAATAACATTGAAAAAGAAATTTACGATTACGGATTTCGTATTTTATAA

Antitoxin

Protein

• Length: 138 a.a.
• Molecular weight: 15251.98 Da
• Isoelectric Point: 4.3830

>AT185129 WP_004247034.1 NZ_CP066833:c1485481-1485068 [Proteus mirabilis]
MLYPAFIEIDEDGSASGWFPDIDGCIFAGDSIEEAHADARSAIDAHFELLSEKGLDIPSPKSQQEHLINSSGDYNNGIWL
LVDVDMDKYDGRAERINITLPHRLLSRIDTIVKTNPEYGSRSGFIATATRKELQKNI

Nucleotide

• Length: 414 bp

>AT185129 NZ_CP088002:c1659116-1658823 [Staphylococcus epidermidis]
ATGGCAGACATGGAGAGTGCACATCACTCAGAGCAAATAAAAACAAATTTAAAATCAAGATTAAATAGAATAGAAGGTCA
AGTAAGAGCAATAAATCGTATGATTGATGATGACGTTTACTGTGATGATGTACTAACTCAAATTAGAGCAACACGTTCAG
CATTGAATAGTGTAGCTACTAAATTACTAGACCATCACATGAAAGGTTGTATTATGGAAAAAATAAACGACGGTGCGCAA
GAAGAAGCAATGGATGAATTGCTCGTGACATTCCAAAAATTAATAAAAGATTAA

Structures

Toxin

• AlphaFold DB: B4ET86

Antitoxin

• AlphaFold DB: B4ET85