Detailed information of TA system TA185126

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 15418..16006
• Replicon: plasmid p2
• Accession: NZ_CP066815
• Organism: Aeromonas caviae strain Aero52

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A3G6Z3M6
• Locus tag: JH254_RS21325
• Protein ID: WP_000438826.1
• Coordinates: 15418..15705 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: N9M5I3
• Locus tag: JH254_RS21330
• Protein ID: WP_001983304.1
• Coordinates: 15692..16006 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH254_RS21305	11646..12549	+	904	Protein_12	replication initiation protein	-
JH254_RS21310	12651..13144	+	494	Protein_13	DNA-binding protein	-
JH254_RS21315	13443..13960	-	518	Protein_14	sel1 repeat family protein	-
JH254_RS21320	14199..15164	+	966	Protein_15	IS30 family transposase	-
JH254_RS21325	15418..15705	+	288	WP_000438826.1	BrnT family toxin	Toxin
JH254_RS21330	15692..16006	+	315	WP_001983304.1	BrnA antitoxin family protein	Antitoxin
JH254_RS21335	16133..18538	+	2406	Protein_18	TonB-dependent receptor	-
JH254_RS21340	18842..19301	+	460	Protein_19	DIP1984 family protein	-
JH254_RS21345	19618..20006	-	389	Protein_20	hypothetical protein	-
JH254_RS21350	20058..20330	-	273	WP_200165043.1	mobilization protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..24192	24192

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11303.72 Da
• Isoelectric Point: 6.0889

>T185126 WP_000438826.1 NZ_CP066815:15418-15705 [Aeromonas caviae]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T185126 NZ_CP088002:c530343-530155 [Staphylococcus epidermidis]
ATGAGCTTACATTTCCAAATTTTGCTGTGGCTTTCTATCTTATTTATCATCGCAGGAACGATATTATTGGTAACAATGCT
TAAAACCAAAAAAGAAGAACGAAAAGAATCTTATCTAGGCTTTACTGTAATTTTCTTAATCTTTGGTTTTGCCATCTTAA
TTTATACTTTTATATTCGGAATTTTATAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12208.84 Da
• Isoelectric Point: 7.2026

>AT185126 WP_001983304.1 NZ_CP066815:15692-16006 [Aeromonas caviae]
MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT185126 NZ_CP088002:c530625-530368 [Staphylococcus epidermidis]
ATGAAATTAAACAAAGCAAATATATTCAATTTGATATTCACCATTTTATTTTTTTCATTTAACATTCTCATAACCTATAA
CGCAAATATAGATTATAAGTTATGGTTAATACCTGGTTTAGCAATTTGTGGTTTTGCTCTTTTTTCAAGCTTGACCTTAG
TAATTATCTATAGTGATTTATTCAGTGAAATATTATTTTTTATCAACATTATCTTAGCACTGTATTACATTTATCCGATT
TTTTATGAATTTGTCTAA

Structures

Toxin

• AlphaFold DB: A0A3G6Z3M6

Antitoxin

• AlphaFold DB: N9M5I3