Detailed information of TA system TA185125

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 4524..5139
• Replicon: plasmid p2
• Accession: NZ_CP066815
• Organism: Aeromonas caviae strain Aero52

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A3G6Z3M6
• Locus tag: JH254_RS21270
• Protein ID: WP_000438826.1
• Coordinates: 4524..4811 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: JH254_RS21275
• Protein ID: WP_200165042.1
• Coordinates: 4798..5139 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH254_RS21245	1..218	+	218	Protein_0	hypothetical protein	-
JH254_RS21250	775..1657	+	883	Protein_1	replication initiation protein	-
JH254_RS21255	1759..2262	+	504	Protein_2	DNA-binding protein	-
JH254_RS21260	2549..3066	-	518	Protein_3	sel1 repeat family protein	-
JH254_RS21265	3305..4270	+	966	Protein_4	IS30 family transposase	-
JH254_RS21270	4524..4811	+	288	WP_000438826.1	BrnT family toxin	Toxin
JH254_RS21275	4798..5139	+	342	WP_200165042.1	BrnA antitoxin family protein	Antitoxin
JH254_RS21280	5237..7640	+	2404	Protein_7	TonB-dependent receptor	-
JH254_RS21285	7943..8402	+	460	Protein_8	DIP1984 family protein	-
JH254_RS21290	8719..9149	-	431	Protein_9	hypothetical protein	-
JH254_RS21295	9167..9433	-	267	WP_006581723.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..24192	24192

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11303.72 Da
• Isoelectric Point: 6.0889

>T185125 WP_000438826.1 NZ_CP066815:4524-4811 [Aeromonas caviae]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T185125 NZ_CP088000:c3834671-3834234 [Stenotrophomonas rhizophila]
TTGGATGACTTCCGCTGCAGCGCTCCACCCCTGGCGAAATGGCTGCTTGAACGCGCACGCCAGAACCAGGCATCCGGCGC
ATCCAGATGCTTCGTTGCCTGCGATGAGAAGCGTCGCGTCGTCGGTTACTACGCGCTCTCAGCCGGCTCGATAGCGCATG
AAGCCGCGCCCGGACGAATCCGCCGCAACATGCCCGATCCCATTCCGGTCATCGTGTTGGGGCGGTTGGCGGTACATGCG
GACAGGGCGGGGCAAGGCATTGGCCAGGGCTTGTTGAAGGATGCGGTGCTGCGTTCGCTGCAGGCGTGCGGTCAGGTCGG
CGCACGGGCGTTGCTCTGCCATGCCATCGATGAGCCCGCCAAGGCGTTCTACCTCAAGCACGGTTTCATCGCTTCACCCA
TTCACCCTCTGACGCTGATGCTGCCCCTTGCGTGCTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13097.90 Da
• Isoelectric Point: 8.4751

>AT185125 WP_200165042.1 NZ_CP066815:4798-5139 [Aeromonas caviae]
MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKIINKNGSLGSHF

Nucleotide

• Length: 342 bp

>AT185125 NZ_CP088000:c3834987-3834718 [Stenotrophomonas rhizophila]
ATGGGAACCGCAAGCAAAGTCATCAATTTCCGGGCCCCTGCCGATAAGCAGGATCTGATCGATCGTGCCGTAGCAATCAG
TGGCGTGAATCGCACCGAGTTCATCCTGGAAGCCGCGTGCGACAGGGCACGCGAGGTGCTGGCCGACCAGACGCAGTTCA
CGTTGAATGCCGAGCAGCTGCAGCGCTTCAATGCGCTGCTGGACGCACCGCTGGAGGACAACCTTGCCCTGCGCCGGTTG
TTGAGCACGCCGGCGCCCTGGGAACGCTGA

Structures

Toxin

• AlphaFold DB: A0A3G6Z3M6