Detailed information of TA system TA185123

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 3345649..3346302
• Replicon: chromosome
• Accession: NZ_CP066812
• Organism: Mycobacterium avium subsp. paratuberculosis strain 42-13-1

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: MAPJR_RS15245
• Protein ID: WP_003872880.1
• Coordinates: 3345649..3346110 (-)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: Q742J1
• Locus tag: MAPJR_RS15250
• Protein ID: WP_003872881.1
• Coordinates: 3346114..3346302 (-)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAPJR_RS15230	3342141..3342527	-	387	WP_003872877.1	hypothetical protein	-
MAPJR_RS15235	3342567..3344150	-	1584	WP_003872878.1	DUF4185 domain-containing protein	-
MAPJR_RS15240	3345146..3345580	-	435	WP_003877359.1	hypothetical protein	-
MAPJR_RS15245	3345649..3346110	-	462	WP_003872880.1	SRPBCC family protein	Toxin
MAPJR_RS15250	3346114..3346302	-	189	WP_003872881.1	antitoxin	Antitoxin
MAPJR_RS15255	3346365..3348737	-	2373	WP_003872882.1	HAD-IC family P-type ATPase	-
MAPJR_RS15260	3348734..3350320	-	1587	WP_003872883.1	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (2-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-51)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16626.19 Da
• Isoelectric Point: 9.5654

>T185123 WP_003872880.1 NZ_CP066812:c3346110-3345649 [Mycobacterium avium subsp. paratuberculosis]
VAKLSGSIDVPLPPEVAWQHASDLSRYKEWLTIHRVWRSTLPDQIDKGTVVESIVEVKGMLNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKVQPKADGSVVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVTVFARSDPSTNGHR

Nucleotide

• Length: 462 bp

>T185123 NZ_CP088000:2238498-2239076 [Stenotrophomonas rhizophila]
GTGAACAGCTCTACATTCACGGCGGTATTTGACGCATGCGTTCTATACCCAGCGAACGTCCGCGACCTGCTGATGCGGCT
GGCCTGCACCCGCTGCTTTCGCGCACGCTGGAGCGGGGACATCCATGACGAATGGATACGCAACCTTTTGGCGAATCGCC
CCGACCTCACGCGGGAACGGCTCGACCGTACAGCACAGCAAATGAATGATGCCGTTGAAGACTGTCTGGTGGCCGACTAC
GCGCCATTGATTGACGGCATTGAGGGTCTGCCTGATCCAGGCGACCGGCACGTTGTAGCGGCGGCCATTCGCTGCGGCGC
CAGCGTCATCGTCACCTACAACCTCAAGGACTTTCCCGCAGCAGCGTTGGAACGCTGGGGGCTCGAAGTACAACACCCGG
ACGAGTTTGTGGAGAACGTATTCGATCTGCACCAAGCCCAGGTATTAGGCGCGGTGCGGAACATGCGCCTCGCGCTCAAG
AGCCCGCCCTTGACTATAGAAGAGCTCTTCAACGTATTGCTTAAGGCAGAACTCGCTCAGACGGTGAAGTGCCTTGAGGG
CTACAAGGCGATGCTCTAG

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6745.42 Da
• Isoelectric Point: 4.4865

>AT185123 WP_003872881.1 NZ_CP066812:c3346302-3346114 [Mycobacterium avium subsp. paratuberculosis]
MGFLDKAKDLLSQNADKVEMAIDKAGEFVDEKTQGKYSDTIHQVQEQAKKATGATETGDQQG

Nucleotide

• Length: 189 bp

>AT185123 NZ_CP088000:2238046-2238501 [Stenotrophomonas rhizophila]
ATGACCCCACAAGTAACCCTTCCCCGCACCCAAGATATCGAAGCGGCCACGACCGCTCAGCGTGAGCTGGCTGCTTATCT
GGCCACCGGATTCGAGACCAAACTGGTCCAACTGGTCGACTCTCAGGGCCGGCTCCACCCGATCCAACTGCCAATGGCGG
CCCTGCAGCTGCTCAGCGAAATCCTATCGGAACTGGCGGATGGCAACGCCGTGAAAATCGTGCCCGTACATGCGGAGCTG
ACTACCCAGGAAGCGGCTGATCTTCTTAATGTATCGCGCCCCTATCTGGTCAAGTTGCTGGAGACCGGCAAGCTTGCCTT
CCACAAGACAGGCAAGCACCGCCGTATTCGCTTCGACGACCTGATGACGTATAAGCAGGCGCGCGATGAGGCAAGTCAGT
CGGCCTTGGACAACCTGGCCGCCGAAGCCCAGGAACTGCGAATGGGTTACGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E2WC18