Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 1975630..1976294 Replicon chromosome
Accession NZ_CP066812
Organism Mycobacterium avium subsp. paratuberculosis strain 42-13-1

Toxin (Protein)


Gene name vapC Uniprot ID Q73YC9
Locus tag MAPJR_RS09140 Protein ID WP_003872398.1
Coordinates 1975887..1976294 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q73YC8
Locus tag MAPJR_RS09135 Protein ID WP_003872399.1
Coordinates 1975630..1975890 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MAPJR_RS09115 1971046..1971708 + 663 WP_003872403.1 TetR/AcrR family transcriptional regulator -
MAPJR_RS09120 1971904..1973361 + 1458 WP_003878220.1 serine/threonine protein kinase -
MAPJR_RS09125 1973563..1974831 - 1269 WP_003872401.1 cytochrome P450 -
MAPJR_RS09130 1974997..1975449 + 453 WP_003872400.1 VOC family protein -
MAPJR_RS09135 1975630..1975890 + 261 WP_003872399.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MAPJR_RS09140 1975887..1976294 + 408 WP_003872398.1 type II toxin-antitoxin system VapC family toxin Toxin
MAPJR_RS09145 1976441..1977985 - 1545 WP_010949452.1 serine/threonine protein kinase -
MAPJR_RS09150 1978222..1978743 + 522 WP_003878215.1 carboxymuconolactone decarboxylase family protein -
MAPJR_RS09155 1978740..1979285 + 546 WP_003878214.1 carboxymuconolactone decarboxylase family protein -
MAPJR_RS09160 1979406..1979936 + 531 WP_003878213.1 TetR/AcrR family transcriptional regulator -
MAPJR_RS09165 1979925..1981094 - 1170 WP_003878212.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-125)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-64)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 14418.47 Da        Isoelectric Point: 4.5642

>T185121 WP_003872398.1 NZ_CP066812:1975887-1976294 [Mycobacterium avium subsp. paratuberculosis]
MTARPAAGVLDTSVFIASESGRQLDEARIPDEVATTVVTLAELHAGVLAATTSNVRAQRLATLESIADMEMLPVDDDAVR
MWARLRIHLAEAGRRMRANDLWIAAIAASRGLPVVTQDDDFAALDGAANLAIIRV

Download         Length: 408 bp

>T185121 NZ_CP088000:c338761-338447 [Stenotrophomonas rhizophila]
ATGCTCTTCATTGAAACGCCCACCTTTACCCGCGCGATCAAAGGACTGATGCCTGACGACGACTACGCCAGGTTCCAGCT
TGAGTTGGCCGCGTCGCCGCTGGTGGGAGATGTAATTGAGGGAACCGGTGGGCTGCGCAAGGTTCGCTTGGGAATCCCAG
GACGTGGGAAGCGTGGCGGTGCGAGGGTCATCTACTACTACATCGCCCCTTCGTCCCGGATCATCCTGCTTCTGGTGTAC
CCAAGGAATGTTCAGGACGCGCTGACCGCAGAACAGAAGATCGCACTCCGATCCATCATTGAGAACTGGAAGTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9635.01 Da        Isoelectric Point: 11.7137

>AT185121 WP_003872399.1 NZ_CP066812:1975630-1975890 [Mycobacterium avium subsp. paratuberculosis]
MSEVASRELRNDTAGVLRRVREGEDVTITINGRAVAMLTPLRPQRRRWLSKAELLSRLRRAQADPGLRQDLATLAGDTTE
DLGPIR

Download         Length: 261 bp

>AT185121 NZ_CP088000:c338443-338147 [Stenotrophomonas rhizophila]
ATGGACAACACCCTTTTTGCCGAACTGATGGGAAGCGTGGCTGAGGCCGATGAGATCCTGAAGGGTAATCAGGTACCGTC
GCGTGAGCTCCATGTCAGCCCCGTGCAAGTGCGCGAGATTCGCCGAGCGACGGGGCTGTCGCAGCAGAAATTCGCGCGCA
TCATCCACGTGGACGTTGGCACGCTGCGAAACTGGGAACAGGGGCGGCGGGACCCCACGGGTCCGGCACGTGCCCTGCTC
CACGTCATCATGAAGAATCCCAAGGAGACGCTTCGGGTGCTGGCGGAGGCGGGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L5MIC8

Antitoxin

Source ID Structure
AlphaFold DB A0A7L5MIC5

References