Detailed information of TA system TA185118

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3411624..3412461
• Replicon: chromosome
• Accession: NZ_CP066809
• Organism: Shigella flexneri 3a strain 888048

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: JGY96_RS17420
• Protein ID: WP_025758752.1
• Coordinates: 3411919..3412461 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: A0A658YW11
• Locus tag: JGY96_RS17415
• Protein ID: WP_005072928.1
• Coordinates: 3411624..3411935 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JGY96_RS17380 (3406737)	3406737..3408333	-	1597	Protein_3394	IS66 family transposase	-
JGY96_RS17385 (3408353)	3408353..3408699	-	347	Protein_3395	IS66 family insertion sequence element accessory protein TnpB	-
JGY96_RS17390 (3408707)	3408707..3409368	-	662	Protein_3396	IS66-like element accessory protein TnpA	-
JGY96_RS17395 (3409452)	3409452..3409604	+	153	Protein_3397	hypothetical protein	-
JGY96_RS17400 (3409594)	3409594..3410208	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
JGY96_RS17405 (3410208)	3410208..3410537	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
JGY96_RS17410 (3410549)	3410549..3411439	+	891	WP_025758754.1	heme o synthase	-
JGY96_RS17415 (3411624)	3411624..3411935	+	312	WP_005072928.1	DUF1778 domain-containing protein	Antitoxin
JGY96_RS17420 (3411919)	3411919..3412461	+	543	WP_025758752.1	GNAT family N-acetyltransferase	Toxin
JGY96_RS17425 (3412517)	3412517..3413452	-	936	WP_024259953.1	tetratricopeptide repeat protein	-
JGY96_RS17430 (3413463)	3413463..3414008	-	546	WP_225620337.1	hypothetical protein	-
JGY96_RS17435 (3414014)	3414014..3414991	-	978	WP_236743660.1	tetratricopeptide repeat protein	-
JGY96_RS17440 (3415358)	3415358..3416728	+	1371	WP_119182053.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (48-155)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19835.99 Da
• Isoelectric Point: 8.6178

>T185118 WP_025758752.1 NZ_CP066809:3411919-3412461 [Shigella flexneri 3a]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLER
QRVSGVLQGSQPSEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHQALPIKGVYLDADPATINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T185118 NZ_CP087999:2737932-2738204 [Geomonas sp. RF6]
ATGAGCTGGAAGATTGAGTTCTCACCTGCTGCAAAGAGGGAACTGGACAGGTTGGATCGTCCTATCGCCCGCCGCATCCT
CAATTTCCTTTCGGAACGTGTTTCGAAGCTCGACGATCCGCGTGCTATGGGTGCAGCCCTCAAAGGGCCGCACCTAGGAG
AGTTCTGGAAATACAGGATCGGAGACTATCGCATAATCGCCAAGATAGAAGATTCACAGCTGCTTATACTGGTGCTCAGG
GTTGGTAACCGGCGCGACATATACGAACGGTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11410.11 Da
• Isoelectric Point: 10.3388

>AT185118 WP_005072928.1 NZ_CP066809:3411624-3411935 [Shigella flexneri 3a]
MLGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT185118 NZ_CP087999:2737724-2737948 [Geomonas sp. RF6]
ATGCTGGCCATAGACTTGCCGGAAGACATACAAATGAGACTTGATCGGCTGGCGAAGGCGACGGGACGGCCGAAAAGTTT
CTATGCACGGGAAGCGATCATCGAATATCTGGATGATCTCGAAGATGCATATCTCGCTGAAAAAGAGCTAGCGGAAATTC
GTGCCGGGAAGGCAAAGACTATTCCGCTGGAGGAAGTGATGCGGGAATATGAGCTGGAAGATTGA

Structures

Antitoxin

• AlphaFold DB: A0A658YW11