Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3370455..3371073 Replicon chromosome
Accession NZ_CP066809
Organism Shigella flexneri 3a strain 888048

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JGY96_RS17205 Protein ID WP_001291435.1
Coordinates 3370855..3371073 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q0T7C3
Locus tag JGY96_RS17200 Protein ID WP_000344797.1
Coordinates 3370455..3370829 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JGY96_RS17190 (3365544) 3365544..3366737 + 1194 WP_024260158.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JGY96_RS17195 (3366760) 3366760..3369909 + 3150 WP_001132472.1 efflux RND transporter permease AcrB -
JGY96_RS17200 (3370455) 3370455..3370829 + 375 WP_000344797.1 Hha toxicity modulator TomB Antitoxin
JGY96_RS17205 (3370855) 3370855..3371073 + 219 WP_001291435.1 HHA domain-containing protein Toxin
JGY96_RS17210 (3371245) 3371245..3371796 + 552 WP_000102569.1 maltose O-acetyltransferase -
JGY96_RS17215 (3371912) 3371912..3372382 + 471 WP_000136192.1 YlaC family protein -
JGY96_RS17220 (3372546) 3372546..3374096 + 1551 WP_025758198.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JGY96_RS17225 (3374138) 3374138..3374491 - 354 WP_000878140.1 DUF1428 domain-containing protein -
JGY96_RS17235 (3374870) 3374870..3375181 + 312 WP_000409911.1 MGMT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T185117 WP_001291435.1 NZ_CP066809:3370855-3371073 [Shigella flexneri 3a]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T185117 NZ_CP087999:c2726337-2725972 [Geomonas sp. RF6]
ATGGTGCTCGTCGACACATCAGTGTGGATTGATCACCTGCGATCCTCCAATGCCGGTCTGGTGGCACTTCTGGAAGAGGG
AACGGTACTCTGCCACCCCTTCGTCGTCGGGGAAGTAGCCTGCGGCAACTTGCGCCAGAGGGAAACGGTTCTCGCACTCC
TCAATGCACTGCCGGTCGCTGCCGAGGCGCAACATCACGAGGTCCTTCTGTTTCTGGAGCGCCACCAGCTCTTTGGAAAG
GGAGTGGGCCTCATTGACATTCATCTCCTGGCCTCAGCCGCCCTCAGCGGCGCACGGATATGGACTCTGGACCAGAGAGT
TGCAGAAGCCGCGGAAACGCTGGGACTTTCATGTAGGCCGGAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT185117 WP_000344797.1 NZ_CP066809:3370455-3370829 [Shigella flexneri 3a]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT185117 NZ_CP087999:c2726531-2726340 [Geomonas sp. RF6]
ATGAGGACCACCATGAACATCGATGACGCACTTCTCGACACCGCATCGAGACTAACGGGGGTAACGGAGAAAACAGCGCT
GGTGAAGCTGGGGCTGCAGGCGCTCATAGCGAGGGAGAGCGCCCGAAGGCTCGCTGAGCTGGGGGGTAGCGAGAAAGAGC
TTGAAGATATCCCACGCAGGAAGTCTCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TPD8

References