Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 158664..159295 Replicon chromosome
Accession NZ_CP066809
Organism Shigella flexneri 3a strain 888048

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag JGY96_RS00745 Protein ID WP_001259385.1
Coordinates 158664..158939 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag JGY96_RS00750 Protein ID WP_000593555.1
Coordinates 158936..159295 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JGY96_RS00730 (153682) 153682..154749 + 1068 WP_000361474.1 HlyD family secretion protein -
JGY96_RS00735 (154746) 154746..157481 + 2736 WP_025758458.1 ribosome-associated ATPase/putative transporter RbbA -
JGY96_RS00740 (157481) 157481..158605 + 1125 WP_001216257.1 ABC transporter permease -
JGY96_RS00745 (158664) 158664..158939 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
JGY96_RS00750 (158936) 158936..159295 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
JGY96_RS00755 (159415) 159415..159816 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
JGY96_RS00760 (159822) 159822..160628 - 807 WP_000173672.1 nickel import ATP-binding protein NikE -
JGY96_RS00765 (160625) 160625..161389 - 765 WP_001136248.1 nickel import ATP-binding protein NikD -
JGY96_RS00770 (161389) 161389..162222 - 834 WP_001008949.1 nickel ABC transporter permease subunit NikC -
JGY96_RS00775 (162219) 162219..163163 - 945 WP_025758457.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T185098 WP_001259385.1 NZ_CP066809:158664-158939 [Shigella flexneri 3a]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T185098 NZ_CP087993:c2481262-2480834 [Xylella fastidiosa subsp. multiplex]
ATGATTGTCTTGGATACCAATGTTGTTTCCGAAGCAATGAAACCCGAGCCGGATGCAGCAGTCCGAACCTGGCTCAACGA
GCAGATGAGCGTCACGTTGTACCTATCCAGTGTGACGTTATCTGAACTGTTGTTTGGAATCGCAGTGTTGCCCACAAGTA
AGCGCAAGGACATGTTGGCTCGTACCCTTGATGGCTTGCTTGATCTGTTCAATGAACGGGTGTTGCCGTTCGATACGGAC
GCGGCACGCCACTATGCTGAACTGGCAGTGAAAGCGAGAAACAATGGGCGCGGCTTTCCCACACCGGATGGCTATATCGC
GGCAATTGCTGCATCACGAGGTTACATAGTAGCTTCCCGCGACACCTCAGCCTATGAGTCAAGTGGTGTGCAAGTCATCA
ATCCGTGGCAATACAGCAAACAAACATAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT185098 WP_000593555.1 NZ_CP066809:158936-159295 [Shigella flexneri 3a]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT185098 NZ_CP087993:c2481517-2481263 [Xylella fastidiosa subsp. multiplex]
ATGGCCATGTTGACAGTGCGTAATTTGCCTGATGAAGTCCATCGCGCCTTGCGGGTTCGAGCCGCCACACACGGCCGCAG
TACAGAAGCGGAAGTACGTGAGATTTTAACGAACATAGTCAAGCCGGATGAGCGTGTTCGCATTGGCGATGCCTTGGCTA
TCCTGGGACGCGAGATCGGACTAAATAACGAGGATTTCACGACCTTTGATAAGGTGCGAGACAAGACGCAGGCCGAGCCG
ATGAGATTTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References