Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4775419..4776035 Replicon chromosome
Accession NZ_CP066806
Organism Escherichia coli strain TCM3e1

Toxin (Protein)


Gene name vapC Uniprot ID A0A176ZIX8
Locus tag JGU77_RS22745 Protein ID WP_001129490.1
Coordinates 4775419..4775793 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1M0CY48
Locus tag JGU77_RS22750 Protein ID WP_001275523.1
Coordinates 4775793..4776035 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JGU77_RS22730 4772922..4773824 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
JGU77_RS22735 4773821..4774456 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
JGU77_RS22740 4774453..4775382 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
JGU77_RS22745 4775419..4775793 - 375 WP_001129490.1 type II toxin-antitoxin system VapC family toxin Toxin
JGU77_RS22750 4775793..4776035 - 243 WP_001275523.1 ribbon-helix-helix domain-containing protein Antitoxin
JGU77_RS22755 4776255..4776473 - 219 WP_001251290.1 ribbon-helix-helix domain-containing protein -
JGU77_RS22760 4777147..4778961 - 1815 WP_000226332.1 hypothetical protein -
JGU77_RS22765 4779377..4780318 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
JGU77_RS22770 4780363..4780800 - 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-115)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-48)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13960.19 Da        Isoelectric Point: 7.0126

>T185089 WP_001129490.1 NZ_CP066806:c4775793-4775419 [Escherichia coli]
MAKRTALFDTNILIDLFSGRIEAKQALEAWPLQNAISLVTWMEVLVGAKKYDQEHRTRIAMSAFNVIGISQDIAERSVAL
RQEYRMKLPDAIILATAQIYGFELVTRNTRDFAGIAGVVTPYEL

Download         Length: 375 bp

>T185089 NZ_CP087992:1599000-1599287 [Xylella fastidiosa subsp. multiplex]
ATGGCTAAGTCCTATCGACTGACACCGCTGGCCGAGGCCGATCTTGAAGAAATCTGGTTCTACACATTCAGGCACTGGTC
GATAGAGCAGGCCGACAGCTACCACCGCAGCCTAGTTGCAGTTTTCGAGGGGCTGGCAGCAGGTACAAAGCAAGGCTGCC
CTTCTGTTTTGCCGGACTTCAATAAATACCTATGTGGTTCGCATGTAGTGTATTTCATGGATTACGCCGATCACTTGGAT
ATTATCCGCATCCTGCATCAGCGGCAGGATACCGAGCGGTACCTATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9290.54 Da        Isoelectric Point: 4.3774

>AT185089 WP_001275523.1 NZ_CP066806:c4776035-4775793 [Escherichia coli]
MSAMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVDQYLENQSKTTISSAFGIWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT185089 NZ_CP087992:1598765-1599007 [Xylella fastidiosa subsp. multiplex]
ATGAGTCGAAACACATCCGTTGCCCTCGGGCCGCACTTCACCAGCTTTATTGATGCCCAAGTCCAGGGCGGTCGCTACGG
TACCGCCAGCGATGTTGTCAGGGCCGGTCTGCGGCTACTTGAGGAACACGAAACAAAGGTGAAGGCACTGCAAGATGCGC
TGAATGTTGGCCACCAATCCGGCGAGCCGCGCCCGTTCGACAGCGAGGTGTTTTTGAGCCGGATGCACACGCAGCATGGC
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A176ZIX8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M0CY48

References