Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4466109..4466606 Replicon chromosome
Accession NZ_CP066806
Organism Escherichia coli strain TCM3e1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JGU77_RS21335 Protein ID WP_001173343.1
Coordinates 4466109..4466282 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JGU77_RS21340 Protein ID WP_000398619.1
Coordinates 4466310..4466606 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JGU77_RS21325 4462779..4464236 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
JGU77_RS21330 4464473..4465990 + 1518 WP_000003800.1 lysine--tRNA ligase -
JGU77_RS21335 4466109..4466282 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JGU77_RS21340 4466310..4466606 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JGU77_RS21345 4466835..4467107 - 273 WP_000405647.1 N-acetyltransferase -
JGU77_RS21350 4467119..4467349 - 231 WP_000371713.1 (4Fe-4S)-binding protein -
JGU77_RS21355 4467530..4469161 + 1632 WP_001216471.1 sensor histidine kinase -
JGU77_RS21360 4469158..4469877 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T185088 WP_001173343.1 NZ_CP066806:c4466282-4466109 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T185088 NZ_CP087992:1325410-1325718 [Xylella fastidiosa subsp. multiplex]
GTGACCATCACAAAGAAAATCAATGTCTCCGAACTGCCAGAGTTCGATGCCGCCGAATACCTGAACAGCGAAGAGGAGGT
AGCCGCGTATCTCACGGCTGTTCTTGAAGAAAACGATCCAGCACTGTTAGCGGCTGCGCTGGGTGACATTGCCCGTTCAC
GTGGCATGTCTCAGATTGCGAAGGATTCTGGCATCACGCGGGAAGCTCTATACAAGGCTCTTCGGCCTGGCAGTGAACCA
CGCTTCGATACGATAAGCCGCGTCTGTACCGCCCTAGGTATACGTCTAGTAGCGCAACCAATGCATTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT185088 WP_000398619.1 NZ_CP066806:c4466606-4466310 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT185088 NZ_CP087992:1325107-1325406 [Xylella fastidiosa subsp. multiplex]
ATGACCTACACGGTAAAACGACTTGAAGGGTTCTCTGACTGGCTAAAGGGCCTAAAGGATGGGCTGGCAAGGCAGCGCCT
CATCAAGCGCTTACGCAAGGTGCAGCTCGGCAACTTTGGAGACGTGCAGCCAGTGGGTGAAGGCGTGTTTGAAATGCGCG
AACACTTCGGACCTGGCTGGCGTATGTACTACGTGCAGCGAGGCAGCTTTCTCATCGTGATGCTCGGCGGCGGCGACAAG
TCAACGCAGCAATCAGACATTCGTCGGGCCATTGAGCTAGCAAAATCTTTAGAGGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References