Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4273795..4274630 Replicon chromosome
Accession NZ_CP066806
Organism Escherichia coli strain TCM3e1

Toxin (Protein)


Gene name yeeV Uniprot ID B1LDL9
Locus tag JGU77_RS20365 Protein ID WP_001094444.1
Coordinates 4273795..4274172 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B7LDN9
Locus tag JGU77_RS20370 Protein ID WP_001285607.1
Coordinates 4274262..4274630 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JGU77_RS20340 4271031..4271408 - 378 WP_000784767.1 hypothetical protein -
JGU77_RS20345 4271477..4272256 - 780 WP_001088803.1 HNH endonuclease -
JGU77_RS20350 4272862..4273008 - 147 WP_001290178.1 hypothetical protein -
JGU77_RS20355 4273093..4273290 - 198 WP_001327226.1 DUF957 domain-containing protein -
JGU77_RS20360 4273310..4273798 - 489 WP_000761685.1 hypothetical protein -
JGU77_RS20365 4273795..4274172 - 378 WP_001094444.1 TA system toxin CbtA family protein Toxin
JGU77_RS20370 4274262..4274630 - 369 WP_001285607.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
JGU77_RS20375 4274710..4274931 - 222 WP_000692350.1 DUF987 domain-containing protein -
JGU77_RS20380 4275018..4275494 - 477 WP_001384029.1 RadC family protein -
JGU77_RS20385 4275509..4275994 - 486 WP_200403398.1 antirestriction protein -
JGU77_RS20390 4276086..4276904 - 819 WP_001175175.1 DUF945 domain-containing protein -
JGU77_RS20395 4276994..4277203 - 210 WP_032150870.1 DUF905 family protein -
JGU77_RS20400 4277233..4277910 - 678 WP_001097301.1 hypothetical protein -
JGU77_RS20405 4278058..4278738 - 681 WP_001282919.1 WYL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fimB 4247820..4313491 65671


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14087.02 Da        Isoelectric Point: 7.3523

>T185085 WP_001094444.1 NZ_CP066806:c4274172-4273795 [Escherichia coli]
MNTLPDTHVREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SVCTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Download         Length: 378 bp

>T185085 NZ_CP087992:1321469-1321765 [Xylella fastidiosa subsp. multiplex]
ATGATTGAATTGAAGCAGACTGACACCTTCCGCAAGTGGCGGGAGAAACTCAAGGATGCGCGCGCCCGCTCGGCCATCGC
CTCGCGCCTCGACCGCTTGGCGTTCGGCCATGTCGGCGACGCCGAGCCAGTAGGGAAAGGTGTCAGCGAGCTTCGCATCA
ACTACGGCCCCGGTTACCGGGTGTATTTCCAGCGGCGTGGCGACACGATCTACTTGCTGCTTTGCGGCGGTGACAAAGGA
TCACAAGCGCGCGACATCAAGACTGCGCTGCACCTGTCTGAACAATGGAGCGAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13689.53 Da        Isoelectric Point: 6.4669

>AT185085 WP_001285607.1 NZ_CP066806:c4274630-4274262 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Download         Length: 369 bp

>AT185085 NZ_CP087992:1321768-1322061 [Xylella fastidiosa subsp. multiplex]
ATGACCGAGAAACTGACGAGCTACGATCCAGCCGAGGACTTGACCACTGACCAGGCCATCGCCGATTTCATGGCAGCTGC
GTTCGGGACGAACGATCCTGCCTACGTTGCCCACGCGCTGGGCGTCGTTGCCCGCGCCAAGGGCATGACGCAGATCGCCA
GCCAGACAGGGCTATCGCGCGAACAGCTCTACCGCTCGTTCAGTGCCGAGGGCAACCCGACGCTGCGCACGACATTGGCC
GTGATGAAGGCGCTCGGGATCGAGCTGTCTGCGAAACCGTCTGGTGTTCACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB B1LDL9

Antitoxin

Source ID Structure
AlphaFold DB A0A1M0JLU8

References