Detailed information of TA system TA185084

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4214028..4214604
• Replicon: chromosome
• Accession: NZ_CP066806
• Organism: Escherichia coli strain TCM3e1

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A2U2VE01
• Locus tag: JGU77_RS20100
• Protein ID: WP_001131965.1
• Coordinates: 4214317..4214604 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: JGU77_RS20095
• Protein ID: WP_106106778.1
• Coordinates: 4214028..4214330 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JGU77_RS20075	4209136..4210356	+	1221	WP_000873756.1	amino acid deaminase	-
JGU77_RS20080	4210624..4212774	+	2151	WP_001314407.1	pyruvate/proton symporter BtsT	-
JGU77_RS20085	4212824..4213027	+	204	WP_000467859.1	YbdD/YjiX family protein	-
JGU77_RS20090	4213038..4213994	+	957	WP_001361361.1	GTPase	-
JGU77_RS20095	4214028..4214330	-	303	WP_106106778.1	BrnA antitoxin family protein	Antitoxin
JGU77_RS20100	4214317..4214604	-	288	WP_001131965.1	BrnT family toxin	Toxin
JGU77_RS20105	4214801..4215967	+	1167	WP_000800823.1	restriction endonuclease	-
JGU77_RS20110	4216012..4216185	+	174	Protein_3936	SAM-dependent DNA methyltransferase	-
JGU77_RS20115	4216179..4216847	+	669	Protein_3937	DUF3387 domain-containing protein	-
JGU77_RS20120	4217062..4217400	+	339	WP_000132633.1	endoribonuclease SymE	-
JGU77_RS20125	4217442..4217606	-	165	WP_000394263.1	DUF1127 domain-containing protein	-
JGU77_RS20130	4217783..4219195	+	1413	WP_000199309.1	PLP-dependent aminotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11223.71 Da
• Isoelectric Point: 7.4021

>T185084 WP_001131965.1 NZ_CP066806:c4214604-4214317 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T185084 NZ_CP087992:c1031908-1031429 [Xylella fastidiosa subsp. multiplex]
ATGCAGATTTCGACACCTCATTCACTCACGGCAGCGCATCGACTTGATGAATTCAATTGCGGTGAGCCATCGCTGGATGA
TTGGCTTAAACGACGCGCACTGACCAACCATTTAAACGGGGCCAGCCGGACCTTCGTGGTTATCGATGCCAACCAATACG
TGCTTGGGTACTACGCTTTGGCAGCCGGGGCTGTAGCGCATCAAGAGGCCACCCGTGCTATCCGACGTAACATGCCTGAT
CCAGTGCCGGTCATGGTGCTCGGAAGGCTGGCTGTGGATACTCGTGCTCAAGGCATCAAACTCGGTGCGGCCTTGCTTAA
AGATACGGTAACTCGTGTGCAGTCCATTGCAGAGAACGCCGGCGTCAGGGCATTGGTAGTCCATGCGTTGAACGAGCGCG
CAAAGCAATTCTACGAGTACTATGGTTTTCGAGCGTCACCAATGCACCTGATGACCTTGATGCTTCCGATTAAATTCTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11550.04 Da
• Isoelectric Point: 9.4646

>AT185084 WP_106106778.1 NZ_CP066806:c4214330-4214028 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDSDVMEWLKRPGKG
YQIRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT185084 NZ_CP087992:c1032183-1031911 [Xylella fastidiosa subsp. multiplex]
ATGCGCGACGCTTCCATCAACTTACGTGCCATGCCTGAGCAGCGCGACTTGATTGACCAGGCTGCTAATCTATTAGGCAA
GAATCGCTCAGACTTCATGCTAGAAGCTGCGTGCGAGCGTGCGAAAGCAATTATCCTGGATCAAGTCTTTTTCAATCTGG
ACGAAGAGAGGTTCCGGCAATTCACTGTGTTGCTTAATGCGCCACAAGACACCAATCCAGGCCTTAATCGCCTGATGGCA
ATTAAGGCTCCGTGGAGCATCAGCAATACGTGA

Structures

Toxin

• AlphaFold DB: A0A2U2VE01