185081

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3728708..3729545 Replicon chromosome
Accession NZ_CP066806
Organism Escherichia coli strain TCM3e1

Toxin (Protein)


Gene name itaT Uniprot ID A0A1M0D5U3
Locus tag JGU77_RS17795 Protein ID WP_000227782.1
Coordinates 3729003..3729545 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID B1LJI1
Locus tag JGU77_RS17790 Protein ID WP_001353405.1
Coordinates 3728708..3729019 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JGU77_RS17765 3723728..3724675 + 948 WP_001239437.1 cytochrome o ubiquinol oxidase subunit II -
JGU77_RS17770 3724697..3726688 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
JGU77_RS17775 3726678..3727292 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
JGU77_RS17780 3727292..3727621 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
JGU77_RS17785 3727633..3728523 + 891 WP_000971327.1 heme o synthase -
JGU77_RS17790 3728708..3729019 + 312 WP_001353405.1 DUF1778 domain-containing protein Antitoxin
JGU77_RS17795 3729003..3729545 + 543 WP_000227782.1 GNAT family N-acetyltransferase Toxin
JGU77_RS17800 3729601..3730445 - 845 Protein_3485 sel1 repeat family protein -
JGU77_RS17805 3730852..3732216 + 1365 WP_001000978.1 MFS transporter -
JGU77_RS17810 3732344..3732835 - 492 WP_001138904.1 nucleotide binding protein YajQ -
JGU77_RS17815 3733003..3733914 + 912 WP_000705836.1 2-dehydropantoate 2-reductase -
JGU77_RS17820 3733877..3734467 + 591 WP_001276309.1 protein deglycase YajL -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(19-96)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19957.31 Da        Isoelectric Point: 9.1763

>T185081 WP_000227782.1 NZ_CP066806:3729003-3729545 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDIIYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCNFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T185081 NZ_CP087978:c5175936-5175019 [Klebsiella variicola subsp. variicola]
ATGGATATACAGGCGCCCTATTATCGGCAGGTTGCTCTGTTAATGCAGGTTTTACCCTATGTTGCCGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACGGCAATCAATCTCTTTATCCGTGATTTCCCACGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCGATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCTAATCTGCAACAACAG
GCGGGTATGAACGCTGTATTACAAGCTAATCGTTCCGATGAGATGCGTGTCGTTGTTACTACCGATAGCGCACAAATAAA
GATAGAGGTCTCACCGGTCGCACGGGGAACATTATATCCACCACAGGAGCGAGAGGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCGGTGGTTTCGTTGCCCGATCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGATGTGAAATTACTTCTTGATGCGCAGGAGCTTAACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTTAGCCATAATCGGCCTCTGGCTGAAGTGCTTAGTCCTCGCTGGAAAGATATTGCTGAACCCTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCTACAGGATGATTGATGCGTTAAAATCC
TGTTTCACGCAACAGGATATCGATTTTTTGCTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAACACG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCGTTAG
ATAAACTGGAAAAGGTGTTGGCCGAGTGGCGCAGTTAG

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11370.09 Da        Isoelectric Point: 10.4825

>AT185081 WP_001353405.1 NZ_CP066806:3728708-3729019 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT185081 NZ_CP087978:c5176700-5175942 [Klebsiella variicola subsp. variicola]
ATGCCATCAAAATTAAACTGGCTGCTGCAAAACAGTGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCGGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACACGCAAAGTGGCTGGTTAAAAAAACGAGGCAACGGCGTATACGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTAGCCGCTCCGGTGTATGTCGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGTCGTTCACACTATCTACAGCTCAAACAAAACCAGTGCTGGTTGAGTATGGAAAATAAAGCGCT
GCTGCCAAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGCAAAAACTGCCGGTGCTGGATGATA
AATACCGCGTAACGCTTGATGTAAAAGGGAAAAGACTCACCGGCAGTGCGCCGGAACTGGCTGCTTACGAGTTGTTGAGC
GCGGTACCGGGGACCCTCTCATTTAACCACGCGGCAGAGCTTTTCCAGGGACTAGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTGCTTTCTGTCAGCCAGTCCGTGCAGACCAAGCGTTTGTATCTCTTTTTCGCCAGTTTTTACGAGCATGGTTGGC
TCAGACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGTAAGCGGCAAATCGTGGTAAATGGCAAGTTCAATGCTCAA
TACCAAATCACTGTGCCTGAACGCTTTCAAAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M0D5U3

Antitoxin

Source ID Structure
AlphaFold DB A0A829G7G3

References