Detailed information of TA system TA185078

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3316870..3317575
• Replicon: chromosome
• Accession: NZ_CP066806
• Organism: Escherichia coli strain TCM3e1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A1M2J6W7
• Locus tag: JGU77_RS15830
• Protein ID: WP_000539524.1
• Coordinates: 3316870..3317256 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JGU77_RS15835
• Protein ID: WP_001280945.1
• Coordinates: 3317246..3317575 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JGU77_RS15810	3312874..3313500	+	627	WP_001295292.1	glutathione S-transferase GstB	-
JGU77_RS15815	3313497..3314612	-	1116	WP_000555050.1	aldose sugar dehydrogenase YliI	-
JGU77_RS15820	3314723..3315106	-	384	WP_000497137.1	biofilm formation regulator BssR	-
JGU77_RS15825	3315319..3316644	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
JGU77_RS15830	3316870..3317256	+	387	WP_000539524.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JGU77_RS15835	3317246..3317575	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
JGU77_RS15840	3317645..3318973	-	1329	WP_106106846.1	GGDEF domain-containing protein	-
JGU77_RS15845	3318981..3321329	-	2349	WP_000950331.1	EAL domain-containing protein	-
JGU77_RS15850	3321506..3322417	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14321.46 Da
• Isoelectric Point: 9.9521

>T185078 WP_000539524.1 NZ_CP066806:3316870-3317256 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSRSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T185078 NZ_CP087978:c4043467-4043150 [Klebsiella variicola subsp. variicola]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAAATAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTATGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT185078 WP_001280945.1 NZ_CP066806:3317246-3317575 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT185078 NZ_CP087978:c4043157-4042870 [Klebsiella variicola subsp. variicola]
ATGAGTAAAAAGATCATTGACTGGGAAACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCGTTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGTGGAATAAAGCACCCACAAATTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: A0A1M2J6W7