Detailed information of TA system TA185066

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2089937..2090721
• Replicon: chromosome
• Accession: NZ_CP066806
• Organism: Escherichia coli strain TCM3e1

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: JGU77_RS09905
• Protein ID: WP_000613626.1
• Coordinates: 2089937..2090431 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: JGU77_RS09910
• Protein ID: WP_000718446.1
• Coordinates: 2090428..2090721 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JGU77_RS09885	2085130..2086227	+	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-
JGU77_RS09890	2086227..2087168	+	942	WP_001317765.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
JGU77_RS09895	2087234..2088877	+	1644	WP_000096449.1	flagellar hook-associated protein FlgK	-
JGU77_RS09900	2088889..2089842	+	954	WP_001212748.1	flagellar hook-associated protein FlgL	-
JGU77_RS09905	2089937..2090431	-	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
JGU77_RS09910	2090428..2090721	-	294	WP_000718446.1	DUF1778 domain-containing protein	Antitoxin
JGU77_RS09915	2090855..2094028	-	3174	WP_000827394.1	ribonuclease E	-
JGU77_RS09920	2094601..2095560	+	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T185066 WP_000613626.1 NZ_CP066806:c2090431-2089937 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T185066 NZ_CP087957:476266-476616 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11053.63 Da
• Isoelectric Point: 10.5423

>AT185066 WP_000718446.1 NZ_CP066806:c2090721-2090428 [Escherichia coli]
MAAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT185066 NZ_CP087957:475979-476260 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: V0T0H9