Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1029913..1030567 Replicon chromosome
Accession NZ_CP066806
Organism Escherichia coli strain TCM3e1

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag JGU77_RS05010 Protein ID WP_000244781.1
Coordinates 1030160..1030567 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JGU77_RS05005 Protein ID WP_000354046.1
Coordinates 1029913..1030179 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JGU77_RS04985 1026001..1027434 - 1434 WP_137490915.1 6-phospho-beta-glucosidase BglA -
JGU77_RS04990 1027479..1027790 + 312 WP_001182965.1 N(4)-acetylcytidine aminohydrolase -
JGU77_RS04995 1027954..1028613 + 660 WP_000250274.1 hemolysin III family protein -
JGU77_RS05000 1028690..1029670 - 981 WP_000886072.1 tRNA-modifying protein YgfZ -
JGU77_RS05005 1029913..1030179 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JGU77_RS05010 1030160..1030567 + 408 WP_000244781.1 protein YgfX Toxin
JGU77_RS05015 1030607..1031128 - 522 WP_001055874.1 flavodoxin FldB -
JGU77_RS05020 1031240..1032136 + 897 WP_000806650.1 site-specific tyrosine recombinase XerD -
JGU77_RS05025 1032161..1032871 + 711 WP_000715222.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JGU77_RS05030 1032877..1034610 + 1734 WP_000813233.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T185065 WP_000244781.1 NZ_CP066806:1030160-1030567 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T185065 NZ_CP087952:3970855-3971193 [Desulfosarcina sp. BuS5]
ATGTCAGTCTATATTCCGAAGCAGGGAGACATAATCGCAATTACATTTGATCCACAAGCTGGGCATGAGCAAAAAGGACG
CCGACCAGCATTGGTTGTCAGCAAGGATCTATTCAATAGAAGCACTGGTTTATCTATTGTGTGCCCAATTACAAATACTG
ACCGTGACTTTCCCTTTCACGTATCAATACCAGAGGCAAGCAAGTTGACCGGTTTCGTCATGGTTGAGCAAGTAAAATCA
GTTGATTTCCGAGCTCGCCGTGTTAAACGTATCGAGCGGCGGAACGACGAACTGTTGTCTGAAGTACTTTCAATATTAGA
TGCTTGTATATATGAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT185065 WP_000354046.1 NZ_CP066806:1029913-1030179 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT185065 NZ_CP087952:3970606-3970854 [Desulfosarcina sp. BuS5]
ATGCTTGCAAAAATTCAAAAATGGGGAAATAGTCAAGGACTTCGCATCACAAAGGCATTGCTTTCAGATGCGCAGATAAA
TGTAGGCGATGACGTCAATATTTTTGTAAAAGACGGCGCCTTAATCGTCACCCCGGCCCAAAAAGTCCGTAAGAAATATA
ATCTCAAGGATCTTGTTGCGAAAATTCCCAAAGGACATCAAACCAAAGAAACCGATTGGGGAGAACCAGTTGGAAAAGAG
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References