Detailed information of TA system TA185064

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 874120..874955
• Replicon: chromosome
• Accession: NZ_CP066806
• Organism: Escherichia coli strain TCM3e1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0A1A9A5
• Locus tag: JGU77_RS04195
• Protein ID: WP_001564063.1
• Coordinates: 874120..874497 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0K5N986
• Locus tag: JGU77_RS04200
• Protein ID: WP_021553056.1
• Coordinates: 874587..874955 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JGU77_RS04165	869247..870395	-	1149	WP_000905920.1	capsule polysaccharide export inner-membrane protein KpsE	-
JGU77_RS04170	870467..871450	-	984	WP_001298261.1	KpsF/GutQ family sugar-phosphate isomerase	-
JGU77_RS04175	872261..872431	-	171	Protein_821	IS110 family transposase	-
JGU77_RS04180	872774..873616	-	843	Protein_822	DUF4942 domain-containing protein	-
JGU77_RS04185	873701..873895	-	195	WP_024181941.1	DUF957 domain-containing protein	-
JGU77_RS04190	873974..874123	-	150	Protein_824	hypothetical protein	-
JGU77_RS04195	874120..874497	-	378	WP_001564063.1	TA system toxin CbtA family protein	Toxin
JGU77_RS04200	874587..874955	-	369	WP_021553056.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JGU77_RS04205	875118..875339	-	222	WP_000692345.1	DUF987 domain-containing protein	-
JGU77_RS04210	875404..875880	-	477	WP_021553055.1	RadC family protein	-
JGU77_RS04215	875896..876375	-	480	WP_001564060.1	antirestriction protein	-
JGU77_RS04220	876641..877459	-	819	WP_001564059.1	DUF945 domain-containing protein	-
JGU77_RS04225	877549..877782	-	234	WP_001278293.1	DUF905 family protein	-
JGU77_RS04230	877788..878465	-	678	WP_001564058.1	hypothetical protein	-
JGU77_RS04235	878616..879296	-	681	WP_001278649.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	papX	872647..946262	73615

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14094.09 Da
• Isoelectric Point: 7.8045

>T185064 WP_001564063.1 NZ_CP066806:c874497-874120 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRSNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T185064 NZ_CP087952:3898018-3898305 [Desulfosarcina sp. BuS5]
ATCTGGTCTCCTCTTGCTGTCGATCGAGCATCAGAAATCGCCGATTACATTGCCCAAGATAAACCATCAGCGGCAGAAAA
ATGGATTGATACAGTATTTTCTAAAGTTGCGCAACTAAACTCGTCTCCTGAAATCGGCCGAATTGTACCTGAAATTAATG
ATAGCCAATTTAAAGAACTGATTTACGGCAATTACCGCATCCTTTATCGTATAGAAAAGGAACAAATATCTATTCTTACG
ATTCGTCATGGCTGCCAAATACTACCAATAAATGAAATTATGGCATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13615.39 Da
• Isoelectric Point: 7.0264

>AT185064 WP_021553056.1 NZ_CP066806:c874955-874587 [Escherichia coli]
VSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGNRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT185064 NZ_CP087952:3897731-3898012 [Desulfosarcina sp. BuS5]
ATGCAAAAACTAAAAATTAATCAAGACATCAGATCCCTTTCAGAAGTCAGGAATGGCATGACAAATTTCATAAAGCAAGT
TCACGATACTAAAAGACCGGTGATAATTATACAGCACGGTAAAGGCGTAGCGGTGCTTTTAGCTGTAAATGAATTTGAAG
CCATGCAAGAAAAAATCGAGCTTCTATCAGATATTCAAACTTCTCTCAACCAACTGGGAAAAGGAGAAGGAATAAGCCAT
AAAGATGCAAAAGAGAAAGTACTCAAGCGAGTTCTAAAATAA

Structures

Toxin

• AlphaFold DB: A0A0A1A9A5

Antitoxin

• AlphaFold DB: A0A0K5N986