Detailed information of TA system TA185062

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 335348..335979
• Replicon: chromosome
• Accession: NZ_CP066806
• Organism: Escherichia coli strain TCM3e1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: JGU77_RS01515
• Protein ID: WP_075861597.1
• Coordinates: 335348..335623 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: U9Y7E1
• Locus tag: JGU77_RS01520
• Protein ID: WP_000593555.1
• Coordinates: 335620..335979 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JGU77_RS01500	330352..331419	+	1068	WP_000361481.1	HlyD family efflux transporter periplasmic adaptor subunit	-
JGU77_RS01505	331416..334151	+	2736	WP_000149118.1	ribosome-associated ATPase/putative transporter RbbA	-
JGU77_RS01510	334151..335275	+	1125	WP_001314210.1	ABC transporter permease	-
JGU77_RS01515	335348..335623	+	276	WP_075861597.1	type II toxin-antitoxin system HicA family toxin	Toxin
JGU77_RS01520	335620..335979	+	360	WP_000593555.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
JGU77_RS01525	336029..336889	-	861	WP_001131792.1	class II aldolase	-
JGU77_RS01530	336921..337190	-	270	WP_000949581.1	HPr family phosphocarrier protein	-
JGU77_RS01535	337180..338688	-	1509	WP_012311797.1	carbohydrate kinase	-
JGU77_RS01540	338681..340039	-	1359	WP_001302220.1	PTS galactitol transporter subunit IIC	-
JGU77_RS01545	340113..340394	-	282	WP_000084021.1	PTS sugar transporter subunit IIB	-
JGU77_RS01550	340391..340864	-	474	WP_012311717.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	325844..335979	10135

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10137.82 Da
• Isoelectric Point: 10.6128

>T185062 WP_075861597.1 NZ_CP066806:335348-335623 [Escherichia coli]
MRTQVQALRKKQKNTLDQIVKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T185062 NZ_CP087952:c2792385-2792050 [Desulfosarcina sp. BuS5]
ATGATTTGTAGTCGATATGATGTGGTTACAGTCCTTTTTCCATTTATTGACAGACCTAAAACGAAAAAACGCCCTGCGGT
TGTACTCAGCAACAAAGAATTTAACAAAAGCAACCACACAATTCTTGCTATGATAACTACTAAAAAAGAGCCGGCATGGC
CGGGAGACTTCCAAATTAGCGATTATACAGATGCCGGATTAAGACTTCCATGTCTGATCCGTTTTAAGTTTTTCACGCTT
GATAACAGGTTGATGCAAAAAAAAATTGGGCATTTAAGCGAAAACGATTCAACTCAAATAGCAAAACATCTATCTTCTTA
TCTGACGGCGTGCTGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13402.27 Da
• Isoelectric Point: 5.1987

>AT185062 WP_000593555.1 NZ_CP066806:335620-335979 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT185062 NZ_CP087952:c2792602-2792375 [Desulfosarcina sp. BuS5]
ATGCAACATGTGAGCAAACTATCTACAAAAGGGCAGGTAACCATACCGGCGGAACTAAGGAAGGCTATAGGTATAGAACC
GGGCGATATGGTAGCCTACGAGTTGCAGGGGAAAAGTGTAAAACTTAAAAGGGTCGAGCCGTTTGACGCAGCTTACCACG
CAGCGGTGTCAAAAACCCTTGAAGAGTGGAACAGCCCTGAAGATGAAGAGGCCTTCAATGATTTGTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9LKZ6