Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 175810..176422 Replicon chromosome
Accession NZ_CP066806
Organism Escherichia coli strain TCM3e1

Toxin (Protein)


Gene name hicA Uniprot ID U9YXE2
Locus tag JGU77_RS00780 Protein ID WP_000833473.1
Coordinates 175810..175995 (+) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1M2IVK0
Locus tag JGU77_RS00785 Protein ID WP_000499742.1
Coordinates 176012..176422 (+) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JGU77_RS00765 171276..172571 + 1296 WP_000985736.1 Fic family protein -
JGU77_RS00770 172679..174217 + 1539 WP_000183978.1 aldehyde dehydrogenase AldB -
JGU77_RS00775 174258..175337 - 1080 WP_000061477.1 type I restriction enzyme HsdR N-terminal domain-containing protein -
JGU77_RS00780 175810..175995 + 186 WP_000833473.1 type II toxin-antitoxin system HicA family toxin Toxin
JGU77_RS00785 176012..176422 + 411 WP_000499742.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
JGU77_RS00790 176534..178513 - 1980 WP_001026863.1 glycoside hydrolase family 127 protein -
JGU77_RS00795 178524..179924 - 1401 WP_000204814.1 MFS transporter -
JGU77_RS00800 180150..180965 + 816 WP_000891820.1 AraC family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6800.89 Da        Isoelectric Point: 11.7053

>T185061 WP_000833473.1 NZ_CP066806:175810-175995 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Download         Length: 186 bp

>T185061 NZ_CP087952:2656752-2657153 [Desulfosarcina sp. BuS5]
ATGCATAATTCTACGGATTACCTGATTGATACGCACTGTTGGCTGTGGTGGCATATTGAGCCCGATCGACTTGCCGGTCA
GACCCTTGAGCTGATTAAAAATGCGGAAACCGTGATTTTCTTTTCCGTTGTCAGTGCCTGGGAGATTATCATCAAGTACA
ATTTAAAAAAACTCAGACTGCCTCTATCTCCTTCCCTTTATATAACCAAACGTCTCGAAATAAGTTACATGGAAGTTATG
CCGGTACATTTTGCCCATGTCCTGCAGGTTGAAAAACTGCCGGATTACCATAAAGATCCATTCGACAGGCTGTTGATTGC
CCAGGCTGTTACAGAGAATTTAACGGTAATTACCTGTGATCATCAATACCGGCAATACGATGTTAAAATAATAGACGGCT
GA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 15242.13 Da        Isoelectric Point: 4.4482

>AT185061 WP_000499742.1 NZ_CP066806:176012-176422 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMDEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Download         Length: 411 bp

>AT185061 NZ_CP087952:2656518-2656748 [Desulfosarcina sp. BuS5]
ATGCTGACAGTGAATACACACGAAGCCAAAACCCAATTATCCCGTTTATTAGTCAGGGTTAGCCATGGCGAAGAAATAGT
TATTGCAAAATCGGGTAAACCGGTTGCCAGACTGGTTCCGGCTAAACAAAATTTTGCCAAACGCAAGCCGGGAATCGATA
AAGGTAAAGCATGGGTGGCCGATGATTTTGATGCGGCCTTACCGGAAACACTGCAGTCATTTTTTGAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9YXE2

Antitoxin

Source ID Structure
AlphaFold DB A0A1M2IVK0

References