Detailed information of TA system TA185058

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1643348..1644696
• Replicon: chromosome
• Accession: NZ_CP066803
• Organism: Pantoea ananatis strain LCFJ-001

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: JGU81_RS07490
• Protein ID: WP_022622806.1
• Coordinates: 1643587..1644696 (+)
• Length: 370 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: JGU81_RS07485
• Protein ID: WP_014605801.1
• Coordinates: 1643348..1643587 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JGU81_RS07470	1638940..1640328	+	1389	WP_014605798.1	MFS transporter	-
JGU81_RS07475	1640351..1642726	+	2376	WP_072403951.1	glycoside hydrolase family 3 C-terminal domain-containing protein	-
JGU81_RS07480	1642790..1643233	-	444	WP_015700266.1	GNAT family N-acetyltransferase	-
JGU81_RS07485	1643348..1643587	+	240	WP_014605801.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
JGU81_RS07490	1643587..1644696	+	1110	WP_022622806.1	HipA domain-containing protein	Toxin
JGU81_RS07495	1644742..1645158	-	417	WP_014605803.1	GFA family protein	-
JGU81_RS07500	1645609..1647477	-	1869	WP_013025825.1	low affinity potassium transporter Kup	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (132-327)

Antitoxin

• Cro/C1-type helix-turn-helix domain (9-55)

Sequences

Toxin

Protein

• Length: 370 a.a.
• Molecular weight: 40875.21 Da
• Isoelectric Point: 7.4551

>T185058 WP_022622806.1 NZ_CP066803:1643587-1644696 [Pantoea ananatis]
MAKLIAWINGERTGTLTLHHNGALHFQYDANWLQHPLARPLSLSLPLQRAAMHSEKVVHFFENLLYPSALIEKDFYPTHP
IKSCEVFAALQKVGHTLPGALSLLPAKRQPVAPDQATHSVFSPADEAQQFDIDNAYLCLSLARELTIPVPDAVLVHENGI
RKLRTERYDRRWRHDGQHLYHLPEEDVCQALGVPLSGRDEQQGGPGIDDIMALLMGSSSAMKDREDLMRCLVFQWLTGIT
RVNAKHLSLLIARGGSFRLAPFKHLLSACPGLSLRTTDRRDFQLGMGLTGPEGKQHHIDAIGPAHFLALAGSVGFSQAAM
RQILAAFIPGLPAAIIKVRDSLPDDYPLTLADAVFSHAMMMLERVRQAI

Nucleotide

• Length: 1110 bp

>T185058 NZ_CP087952:c1425234-1424980 [Desulfosarcina sp. BuS5]
ATGCGAGTTGTCAATTTTAGTGAAGCCAGAAACAATTTAAAATCCGTTTTAGATCAAGTCGTTGATGATGCTAACTACAC
TATAATCACTCGGCGGGATTCAGAAAATGCGGTGGTAATGTCCTTAGACACCTTCAATAGTTATATGGAGACATTCCATC
TTCTCAGATCGCCTGCTAATGCTGCTCATCTATCAAAATCAATTGAACAATATAAAAATGGAAAAGTTCAAGAAAAGGAA
TTGATAGTTGAGTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8773.03 Da
• Isoelectric Point: 7.2012

>AT185058 WP_014605801.1 NZ_CP066803:1643348-1643587 [Pantoea ananatis]
MIYSPRQLANQVRLLRQQNGWTQVELAKKVGLKQATLSHFENAPDTTSLATLFKILQALDLALLLHENSASSAPAEDAW

Nucleotide

• Length: 240 bp

>AT185058 NZ_CP087952:c1424987-1424727 [Desulfosarcina sp. BuS5]
TTGAGTAGGATACTTGCTTGGACTGATGAAGCATGGCAAGGATATCTCTATTGGCAATCGCAAGATAGAAAAACACTTAA
GCGAATTAATAAACTGATTGAAGATACGAAAAGAGAACCTTTTGTGGGAATAGGAAAACCTGAACCATTGCGTGAAAATT
TGTCTGGGTTTTGGTCTCGTCGAATAGATGACACAAATCGTTTAGTATATGCTGTCAATGACAATTTTATCGCTATTATT
TCATGTAGATATCACTATTAA