Detailed information of TA system TA185033

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2870953..2871406
• Replicon: chromosome
• Accession: NZ_CP066794
• Organism: Erwinia amylovora strain 17-2187

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: JGC41_RS13170
• Protein ID: WP_013035888.1
• Coordinates: 2870953..2871189 (-)
• Length: 79 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: D4HZA2
• Locus tag: JGC41_RS13175
• Protein ID: WP_004159527.1
• Coordinates: 2871179..2871406 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JGC41_RS13150	2866468..2867346	-	879	WP_004159509.1	Fe(3+)-hydroxamate ABC transporter substrate-binding protein FhuD	-
JGC41_RS13155	2867361..2868155	-	795	WP_004159512.1	Fe3+-hydroxamate ABC transporter ATP-binding protein FhuC	-
JGC41_RS13160	2868204..2870420	-	2217	Protein_2552	ferrichrome porin FhuA	-
JGC41_RS13165	2870748..2870966	+	219	WP_004159522.1	hypothetical protein	-
JGC41_RS13170	2870953..2871189	-	237	WP_013035888.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JGC41_RS13175	2871179..2871406	-	228	WP_004159527.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
JGC41_RS13180	2871727..2872014	+	288	WP_004159528.1	SymE family type I addiction module toxin	-
JGC41_RS13185	2872078..2872581	-	504	WP_048901601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-78)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-75)

Sequences

Toxin

Protein

• Length: 79 a.a.
• Molecular weight: 9135.79 Da
• Isoelectric Point: 9.0876

>T185033 WP_013035888.1 NZ_CP066794:c2871189-2870953 [Erwinia amylovora]
ISYELLFDPWALKEWKKLGATVREQFKKKLAEVLVNLQVESARLRDHPDCCKIKLKSSGYRLVYQVQDEQLVMLVISV

Nucleotide

• Length: 237 bp

>T185033 NZ_CP087880:c3280125-3279835 [Pseudocitrobacter corydidari]
ATGATCTATGAACTGGCCTTTGACCCGAGGGCGTTAAAAGAGTGGCAAAAGTTAGGTGCTACTGTCCGGGAGCAATTTAA
AAAGAAACTGAAGGTAGTTTTGCTTCATCCGCGCAATGAAGCTGACAGGTTACGAACGTTTCCCGATTGCTACAAAATCA
AACTAAAGGCTTCCGGGTTCAGGCTGGTTTACCAGGTGCAAGAGGCCCAGGTCACGGTACTGGTGATAGCGATTGGCAAG
CGGGAGAAATCTGCCGTTTATCAGGATGCTGACAAACGGCTTGAAGACTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8435.90 Da
• Isoelectric Point: 7.9766

>AT185033 WP_004159527.1 NZ_CP066794:c2871406-2871179 [Erwinia amylovora]
VRLDDKLKSKACAVLDELDVSPTEAVRLLFQYIAENERLPLKALTVSDEKDALLKTVRERLASLQKGIKVTLDKL

Nucleotide

• Length: 228 bp

>AT185033 NZ_CP087880:c3280357-3280115 [Pseudocitrobacter corydidari]
ATGGCGACCATCAACGTCAGACTTGACGATCAACTCAAAAAAGAGGCATACGCCGTGCTGGAAAAACTCAACATCACTCC
CACGGAAGCTGTGCGATTACTGTTTCAGTATTTGGCGGATAATGGGCGGATGCCGGTAAAAAACGTCACCATCAGCGACA
GTGAAGAAGCGCTGCTGCATTCTGTCAGGGAAAGATTGGCCGCTCCGCAAGAGGCCGTTAAGGTCAGGTTGGATGATCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A831A410