Detailed information of TA system TA185030

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1083827..1084454
• Replicon: chromosome
• Accession: NZ_CP066794
• Organism: Erwinia amylovora strain 17-2187

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: D2T444
• Locus tag: JGC41_RS05005
• Protein ID: WP_004156337.1
• Coordinates: 1083827..1084045 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: JGC41_RS05010
• Protein ID: WP_013035913.1
• Coordinates: 1084065..1084454 (-)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JGC41_RS04975	1079813..1080151	+	339	WP_004156319.1	P-II family nitrogen regulator	-
JGC41_RS04980	1080169..1081470	+	1302	WP_004172214.1	ammonium transporter AmtB	-
JGC41_RS04985	1081545..1082405	-	861	WP_004156324.1	acyl-CoA thioesterase II	-
JGC41_RS04990	1082630..1083187	+	558	WP_004156333.1	YbaY family lipoprotein	-
JGC41_RS04995	1083210..1083527	-	318	WP_004156334.1	MGMT family protein	-
JGC41_RS05005	1083827..1084045	-	219	WP_004156337.1	hemolysin expression modulator Hha	Toxin
JGC41_RS05010	1084065..1084454	-	390	WP_013035913.1	Hha toxicity modulator TomB	Antitoxin
JGC41_RS05015	1084601..1084954	-	354	WP_004156342.1	hypothetical protein	-
JGC41_RS05020	1085726..1085872	-	147	WP_004156349.1	type B 50S ribosomal protein L36	-
JGC41_RS05025	1085860..1086141	-	282	WP_004156350.1	type B 50S ribosomal protein L31	-
JGC41_RS05030	1086289..1089435	-	3147	WP_004161886.1	multidrug efflux RND transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8627.06 Da
• Isoelectric Point: 8.9007

>T185030 WP_004156337.1 NZ_CP066794:c1084045-1083827 [Erwinia amylovora]
MTDKLLTKTDYLMRLRRCRSIDTLERVIEKNKYELSDDELAVFYSAADHRLAELTMNKLYDKVPVAVWKFVR

Nucleotide

• Length: 219 bp

>T185030 NZ_CP087880:c2802063-2801695 [Pseudocitrobacter corydidari]
ATGAAATATCAACCTGCAACAACCCTGCGGGCTGCGAAGCCATGCTTGTCGCCCGTGGCTGTCTGGCAAATGCTACTCAC
GCGTCTGCTGGAACAACATTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATTG
ATGCTGGTATTACCCTGGCCGATGCAGTTAACTTCCTGGTAGATAAGTATGAACTGGTTCGTATCGATCGCAGAGGATTT
AGCTCACAGGGACCAGCATCATATTTGACCGTGACAGATATCCTTCATGCCAGGAGAGCATGCGGATTAATGAACAGTTA
TTCGTATCGGGAAGTAAGTAATATTGTTCTTAGCCGATCGCGGCAGTGA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 14965.70 Da
• Isoelectric Point: 4.7040

>AT185030 WP_013035913.1 NZ_CP066794:c1084454-1084065 [Erwinia amylovora]
MDEYSPKRHDIAQLKFLCENLYDESLATLGDSHHGWVNDPTSTSNLQLNDLIEHIAAFTMNYKIKHIEDSDLISQIDEYL
DDTFMLFSNYGVNNLDLQRWQKSAKRLFNIFAKECVMSQIQSSHSFSSP

Nucleotide

• Length: 390 bp

>AT185030 NZ_CP087880:c2802439-2802083 [Pseudocitrobacter corydidari]
ATGAATAATCAATCAGAATCCGTTAACAAACCGGAGAATCTTACCTGCCAGCAGTGGGGACTAAAGCGCACGATCAAACC
CTGTTTTGGCGCTCGTCTGGTACAGGAAGGCAACCTGGTGCATTTTCTCGCTGACCGAGCTGGATTCAAAGGTGCTTTCA
GCGACGATGACGCGCTACATCTGGATCAGGCCTTTCCGTTGATACTTAAGCAACTGGAACTGATGCTCACCAGCGGAGAA
CTCTCTCCCGGGCATCAGCACTGCGTCACGCTCTATCACAATGGACTCACGTGCGAAGCCGATACTCTTGGTAGTTGCGG
CTACGTATACATTGCCATTTATCCCTCTCAATATTAA

Structures

Toxin

• AlphaFold DB: A0A831EPQ0