Detailed information of TA system TA185023

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4487..5259
• Replicon: plasmid unnamed1
• Accession: NZ_CP066789
• Organism: Burkholderia glumae strain FDAARGOS_921

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: I6G78_RS00035
• Protein ID: WP_080942736.1
• Coordinates: 4765..5259 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A2J9HHX2
• Locus tag: I6G78_RS00030
• Protein ID: WP_045678699.1
• Coordinates: 4487..4768 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6G78_RS00010	1308..2027	+	720	WP_045678698.1	hypothetical protein	-
I6G78_RS00015	2074..3153	+	1080	WP_017432519.1	MBL fold metallo-hydrolase	-
I6G78_RS00020	3310..3591	-	282	WP_042967384.1	virulence factor	-
I6G78_RS00025	3842..4299	-	458	Protein_4	transposase	-
I6G78_RS00030	4487..4768	+	282	WP_045678699.1	DUF1778 domain-containing protein	Antitoxin
I6G78_RS00035	4765..5259	+	495	WP_080942736.1	GNAT family N-acetyltransferase	Toxin
I6G78_RS00040	5352..5822	+	471	WP_080942737.1	ProQ activator of osmoprotectant transporter prop	-
I6G78_RS00045	5912..8833	-	2922	WP_017432540.1	conjugative relaxase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..196019	196019

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-84)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17493.08 Da
• Isoelectric Point: 8.6141

>T185023 WP_080942736.1 NZ_CP066789:4765-5259 [Burkholderia glumae]
MTGWLAPEPLGDAHGLDDFDSGVASLDTWLKRRAFANQRSGASRTFVATRDGRVGAYYALASGAVTLEVAPGRFRRNMPD
PIPVVVLGRLAVDRAHQGNGVGRALVRDVGLRVLHAAAAIGIRGLIVHALTDSAKAFYERVGFEASPIDPMLLLITLADL
EHAL

Nucleotide

• Length: 495 bp

>T185023 NZ_CP087879:c2822492-2822232 [Agromyces marinus]
GTGACCCGCGCGCTCGCGTTCGATCAGAACGGCTGGGAGGACTACGTCTACTGGCAGACGCAAGACCGCAAGACGCTCAG
ACGGATCAACGTCCTGATCGCCGACGTGCTCCGTGACCCGTTCGCCGGCATCGGGAAGCCGGAGCCGCTCAAGCACATCC
TTTCGGGTGCGTGGTCTCGCCGCATCGACGAGACGAATCGGCTCGTCTACTACGTCACGGACGACCACATCGTCATCCTG
CAAGCCCGCGACCACTATTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10112.39 Da
• Isoelectric Point: 7.0104

>AT185023 WP_045678699.1 NZ_CP066789:4487-4768 [Burkholderia glumae]
MTSTTPRDTLNLRIRPDDRSLIDRAAAAAHKTRTDFVLDAARAAAEQTLLDQVVLSAEPQAYAAFLARLDAPAQPNAALR
QTMQTRAPWDPAA

Nucleotide

• Length: 282 bp

>AT185023 NZ_CP087879:c2822740-2822489 [Agromyces marinus]
ATGTCGGCAATCACCGCGACCGAGGCGCGCAAGAGCCTGTTCGGCCTGATCCAGCAGGTCAACGACGATCACACCGCAGT
CGAGGTCGTCTCTCGACACGGCAACGCCGTGATCATGTCGAAGGACGACTACGACGCGATCACGGAGACCGCTCACTTGC
TGCGCAACCCTGCCAACGCCGAGCGGCTCCTCGACGCGATCGAGCGGGCGCGTCGCGGCGAGTTCGAGCAGCACGACCTC
CTCGAAGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2J9HHX2