Detailed information of TA system TA185022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 44803..45464
• Replicon: plasmid plas-001
• Accession: NZ_CP066787
• Organism: Martelella lutilitoris strain ZRK001

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JET14_RS20900
• Protein ID: WP_200338267.1
• Coordinates: 44803..45207 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JET14_RS20905
• Protein ID: WP_200338268.1
• Coordinates: 45204..45464 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JET14_RS20885	43299..43877	-	579	WP_200338302.1	hypothetical protein	-
JET14_RS20890	43954..44253	-	300	WP_132312817.1	HigA family addiction module antidote protein	-
JET14_RS20895	44266..44541	-	276	WP_200338266.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JET14_RS20900	44803..45207	-	405	WP_200338267.1	type II toxin-antitoxin system VapC family toxin	Toxin
JET14_RS20905	45204..45464	-	261	WP_200338268.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JET14_RS20910	45525..46082	-	558	WP_200338269.1	hypothetical protein	-
JET14_RS20915	46209..48317	-	2109	WP_200338270.1	ParB/RepB/Spo0J family partition protein	-
JET14_RS20920	48461..48742	-	282	Protein_53	antirestriction protein	-
JET14_RS20925	49021..49611	+	591	WP_200338271.1	TetR/AcrR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..166348	166348

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (15-55)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14049.30 Da
• Isoelectric Point: 5.0855

>T185022 WP_200338267.1 NZ_CP066787:c45207-44803 [Martelella lutilitoris]
MSRLYMLDTNIVSDLARNPAGAVVGHIADVGQDMICVSIITAAELCYGCERKGSAKLTAQVEAILGGMQILALDAPAHAR
YGRIRAGLEAAGTPIGPNDLLIAAHALAVDAVLVTANTREFLRVPGLEVENWIA

Nucleotide

• Length: 405 bp

>T185022 NZ_CP087865:c2352238-2351936 [Moraxella bovis]
ATGACCAAGTCTGATAAATTAAAAGCCAAATTGTATGGCGATGTACGAACTTTTGTATTTAGTGATTTGGTTACTTTATT
ATCGCAATTAGGTTATGAAATGCACGAACGGGCAGGTTCTCGTGTGGTATTTGTTCATTGTGATGATAATAGCGACCGCA
TTCATTTGCATAAACCCCACCCCGAAAATACCATTAAAGGTGGAGCATTAAAAGCGGTCAAAGCCTATTTGTCCGAAAAA
GGCTATTTTGATGAATTTAATGATGATGTGTTAAATCAGGAGCTTAAAAATGATGACCTATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9676.08 Da
• Isoelectric Point: 5.1171

>AT185022 WP_200338268.1 NZ_CP066787:c45464-45204 [Martelella lutilitoris]
MPQPQASEAREAKLFRNNKSQAVRIPADFELPGDRVLIHREGSRLVIEPAPNKNLLNILARLKPLGPDDQFPDVDETLLP
AREIDL

Nucleotide

• Length: 261 bp

>AT185022 NZ_CP087865:c2351949-2351614 [Moraxella bovis]
ATGATGACCTATAAAGGCTATGTTGGCGAATTTACGCTTGATAATGATGATGAAATATTTTATGGTAAACTTATTGGCAT
TAATCAGCTTGTTACTTTTGAAGCAGACAATGCCCATGATTTAAAACAAGCCTTTTATGACAGCGTGGACGATTATTTGG
CGTTTTGTGCTGAACAAGGCATAGAGCCTGATAAGCCTTTTAAGGGTTCATTTAATGTGCGTATTGATAGCGAATTGCAC
CGCCGTGCTTATATGGCAAGCCGTGACAGTAGTTTAAATGCGTTTGTAGGACAAGCCATTAGTGAAAAGCTAAATAGAAT
GGCTGTATCAATATAA