Detailed information of TA system TA185012

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1341110..1342852
• Replicon: chromosome
• Accession: NZ_CP066772
• Organism: Ralstonia pickettii strain K-288

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U3G8X9
• Locus tag: RP6297_RS22105
• Protein ID: WP_009239757.1
• Coordinates: 1341950..1342852 (-)
• Length: 301 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U3G8Y0
• Locus tag: RP6297_RS22100
• Protein ID: WP_009239756.1
• Coordinates: 1341110..1341937 (-)
• Length: 276 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RP6297_RS22080	1336217..1337719	-	1503	WP_009239752.1	aldehyde dehydrogenase	-
RP6297_RS22085	1337736..1338539	-	804	WP_009239753.1	aspartate dehydrogenase	-
RP6297_RS22090	1338572..1340311	-	1740	WP_009239754.1	thiamine pyrophosphate-binding protein	-
RP6297_RS22095	1340304..1341107	-	804	WP_037028679.1	SDR family oxidoreductase	-
RP6297_RS22100	1341110..1341937	-	828	WP_009239756.1	IclR family transcriptional regulator	Antitoxin
RP6297_RS22105	1341950..1342852	-	903	WP_009239757.1	alpha/beta hydrolase	Toxin
RP6297_RS22110	1342857..1343393	-	537	WP_004634817.1	cupin domain-containing protein	-
RP6297_RS22115	1343426..1344199	-	774	WP_009239758.1	SDR family oxidoreductase	-
RP6297_RS22120	1344192..1345259	-	1068	WP_009239759.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-
RP6297_RS22125	1345339..1345644	-	306	WP_009239760.1	hypothetical protein	-
RP6297_RS22130	1345916..1346383	-	468	WP_009239761.1	GNAT family N-acetyltransferase	-
RP6297_RS22135	1346864..1347115	-	252	WP_004634828.1	DUF2798 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (51-288)

Antitoxin

• Transcription regulator IclR, C-terminal (137-258)

Sequences

Toxin

Protein

• Length: 301 a.a.
• Molecular weight: 31753.95 Da
• Isoelectric Point: 5.7757

>T185012 WP_009239757.1 NZ_CP066772:c1342852-1341950 [Ralstonia pickettii]
MNAQAVMSSHPFRVADALAVLGAGFVERTVAINDHGDRIGYRQWGERGPLVVLLHGISSSAASWLPCAQVLSHNMRVLAW
DAPGYGSSTPLADVAPRAADYAGRLQAWLAALDVTPDVIVGHSLGALMASAYVAAAPAAMQPKCLMLLNPAQGYGQPGQE
DQSRSVQAQRLATLDQLGIDGMAESRHAHLLRPNATDEERAWVRWNMKRLNDGGYRQAVAMLSGDDIGAYLSKRPASTPA
QIACGDVDGITPPEGCEALAKMFDLPFALVPSAGHASYIDAPDAVAGWIEHAVLSQSQNA

Nucleotide

• Length: 903 bp

>T185012 NZ_CP087863:c1918007-1917606 [Moraxella bovis]
ATGTTTAGTTTTATGCTGGATACCAATATCTGCATTTATGTGATAAAAGAACGCCCCATAAAAGCATTAAACAAATTTAA
CCAATATGCAGGGCGGATTTGCGTAAGCAGTATCGTGGCAAGCGAGTTGTATTTTGGGGCATACAACAGCCAATTTATGG
ATAAAAATCTACATCAAGTCGAAGATTTTTTATCACGTTTGACGGTGCTTGATTATACCGCAGAATGCTCGCCCCATTAC
GGCGAGATTTGTGCTGATTTGACCAAGCAGGGCAAGCTCATTAGTGAAAATGACATTCATATCGCCTCTCATGCACGGGC
ATTGGGGCTGACCTTGATTACCAATAATACCGATGAATTTAAACGAGTGTCAGGGCTTAGGATTGGTAATTGGGCGATTT
GA

Antitoxin

Protein

• Length: 276 a.a.
• Molecular weight: 30684.56 Da
• Isoelectric Point: 6.5031

>AT185012 WP_009239756.1 NZ_CP066772:c1341937-1341110 [Ralstonia pickettii]
MSNDLIPEDVQEKYIVPGLERGLRLLCEFSHKDRVLSAPELARRLKVPRSTVFRLLTTLEVMGFIERVDGGRDFRLGMAV
LRLGFEYLASLELTELGRPLLDRLRDDIHYPCNLVVRDGRSIVYVAKSVAPTPFTSSVNVGTRLPAHATVLGRVLLADLS
LAELRQLYPEPQLEVFSANTPRTVEELFVMVQRDREHGYVLQEGFFEANISTIAAPVLDRAGKVVAALGTTIPSPRIEAA
QLDLMIDKVRAAAGELSYLLDYRPASGKVVNLFRE

Nucleotide

• Length: 828 bp

>AT185012 NZ_CP087863:c1918255-1918022 [Moraxella bovis]
ATGCAAACCAGTGTTTTTTTTAATAATCGCACCCAAGCGGTTCGCATTCCTGCCAAATTTCGCTTTGATGACGATGTCAA
AGCCGTCATGATTAGAAAAGTGGGCAATGAGCGGATTTTGACCCCAATTGATGGTGTGTGGGACAGCTTTTTTTTGAGTG
ATGAGCGAGCGAGTGATGATTTTATGGCAAACCGCCCGACCATGCCAAAAAGCGAGCGATTGACTTTTGACTGA

Structures

Toxin

• AlphaFold DB: U3G8X9

Antitoxin

• AlphaFold DB: U3G8Y0