Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 80213..80772 Replicon plasmid unnamed1
Accession NZ_CP066760
Organism Escherichia coli O157:H7 strain ECP19-198

Toxin (Protein)


Gene name relE Uniprot ID O82928
Locus tag JG533_RS27395 Protein ID WP_000421248.1
Coordinates 80497..80772 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag JG533_RS27390 Protein ID WP_001178089.1
Coordinates 80213..80497 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG533_RS27350 75404..75964 + 561 WP_000840472.1 fertility inhibition protein FinO -
JG533_RS27355 76097..76309 + 213 WP_001302189.1 ANR family transcriptional regulator -
JG533_RS27360 76554..77015 + 462 WP_001233853.1 thermonuclease family protein -
JG533_RS27365 77061..77270 + 210 WP_001302200.1 hemolysin expression modulator Hha -
JG533_RS27370 77308..77646 + 339 WP_000766796.1 hypothetical protein -
JG533_RS27375 77886..78140 + 255 WP_000083831.1 replication regulatory protein RepA -
JG533_RS27380 78376..78450 + 75 WP_001370046.1 RepA leader peptide Tap -
JG533_RS27385 78443..79300 + 858 WP_000130945.1 incFII family plasmid replication initiator RepA -
JG533_RS27390 80213..80497 + 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
JG533_RS27395 80497..80772 + 276 WP_000421248.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JG533_RS27400 80867..81073 + 207 WP_001105064.1 hypothetical protein -
JG533_RS27405 81173..81388 - 216 Protein_91 transposase -
JG533_RS27410 81432..81998 - 567 Protein_92 IS3-like element IS600 family transposase -
JG533_RS27415 81998..82414 - 417 Protein_93 IS91 family transposase -
JG533_RS27420 82414..82599 - 186 WP_001302179.1 hypothetical protein -
JG533_RS27425 82776..84986 + 2211 WP_000592771.1 catalase/peroxidase KatP -
JG533_RS27430 85030..85419 + 390 WP_001172748.1 cytochrome b562 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - hlyB / hlyD / toxB / toxB / toxB / toxB / espP / stcE / exeE / exeG / hlyC / hlyA 1..118266 118266


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10735.42 Da        Isoelectric Point: 9.9324

>T184994 WP_000421248.1 NZ_CP066760:80497-80772 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T184994 NZ_CP087859:c2257072-2256884 [Moraxella bovis]
ATGCCCCCAAGTAGCGATGAGATGATAAAGCGAATTACCGAAGACGGTTGGTATTTTGTAAAACAAAAAGGCTCTCACAA
GCACTACAAACACCCAACCAAAGAAGGTAAAGTAACAATCCCAAACGGTCGTGAAAACCTACCCAAAGGAACGCTAAAAA
GCATCATCAAACAAGCAGGGTTGGATTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT184994 WP_001178089.1 NZ_CP066760:80213-80497 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT184994 NZ_CP087859:c2256821-2256414 [Moraxella bovis]
ATGTTATTTCCCATTGCCATTGAAAAACCTGCCAATGAAAACGAATGTTATGGCGTGATTGTGCCAGACATTGCAGGGTG
TTTTAGTGCAGGCGATACACCTGATGAAGCCATTAAGAATGCTTATGAAGCCATCAGTTTGCATTTGGAAGGCATGGCAG
AAGATGGCGATACACTACCTACCGCCAAGCCCATTTTTTATCATCAAGACAATAGCGAATTTGACGGTATGTTGTGGGCA
GTGGTGGACGTGGATATGTCTGCCTTTATGGGTAAGGCGGAGAAAATCAACGTTACCTTGCCAAGCCTACTCATCACCAA
AATTGACGAAAAAGTCTTGTCTCATAAATCGCTATACAAAAGCCGTTCTAATTATTTGGCACAATTGGCGATGGCAGATT
TGGGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z6FAZ0

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References