Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 9025..9550 Replicon plasmid unnamed1
Accession NZ_CP066760
Organism Escherichia coli O157:H7 strain ECP19-198

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag JG533_RS27000 Protein ID WP_001159868.1
Coordinates 9245..9550 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag JG533_RS26995 Protein ID WP_000813634.1
Coordinates 9025..9243 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG533_RS26965 4308..4538 + 231 WP_010891288.1 hypothetical protein -
JG533_RS26970 4659..5399 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
JG533_RS26975 5684..6661 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
JG533_RS26980 7069..7269 - 201 WP_000708307.1 hypothetical protein -
JG533_RS26985 7266..7886 - 621 WP_001248529.1 hypothetical protein -
JG533_RS26990 7883..8566 - 684 WP_010891291.1 DNA-binding protein -
JG533_RS26995 9025..9243 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JG533_RS27000 9245..9550 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
JG533_RS27005 9551..10357 + 807 WP_000016989.1 site-specific integrase -
JG533_RS27015 12255..12593 + 339 WP_071525396.1 RepB family plasmid replication initiator protein -
JG533_RS27020 13181..14347 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - hlyB / hlyD / toxB / toxB / toxB / toxB / espP / stcE / exeE / exeG / hlyC / hlyA 1..118266 118266


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T184992 WP_001159868.1 NZ_CP066760:9245-9550 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T184992 NZ_CP087859:c1991674-1991339 [Moraxella bovis]
ATGTGCGAATTAATGTATGAATTAGAATTTGACGAGCGAGCCTTAAAAGAATGGCAAAAACTGGGCGACACCATAAAAAC
GCAGTTTAAAAAGAGACTGGCTAAGGTGCTAAAAAATCCGCACATCATCGCCAATCGCCTAAGCGGTGGACAAAATTTAT
ACAAAATCAACTTCCGCTCGGCAGGTTATCGCCTTGTGTATCAAGTTGAAGATGATAAAGTGGTGGTGTTTGTCATCGTG
ATGGGTAAGCGTGAGAAAAATGCCGTCTATGACACCGCCGAAGAATGGCTTGCTGAACGTCAATCGGACAATCAAGACAC
ACAATCTGTCAAATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT184992 WP_000813634.1 NZ_CP066760:9025-9243 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT184992 NZ_CP087859:c1991916-1991674 [Moraxella bovis]
ATGGCAAATATCGTGGCTCGTGTGGACGATGACCTAAAAGCCCAATCATTTTTGGTACTGCAAGAACTTGGCGTGACACC
GTCTGAGCTTATCCGCCAAACCTTGCAATATGTGGCTTATCACAAAAAATTACCCTTTAAGACGGTGGTTTTGACCGATG
AAGACAGTGAGCTTTTAGCCCTTGCCAAAGAACGCCTTGATAATCCTGCCCCACGCATCAAAGTCAGTTTGGACGAGCTT
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References